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发货周期:现货
博士德生物
cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).
96T
货号 | EK0563 |
---|---|
产品名称 | Human CT-1 ELISA Kit |
产品描述 | For quantitative detection of human CT-1 in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). |
免疫原 | E.coli, S2-A201 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <10pg/ml |
检测范围 | 156pg/ml-10000pg/ml |
基因名称 | CTF1 |
基因全名 | cardiotrophin 1 |
Uniprot ID | Q16619 |
指标别名 | Cardiophin 1|Cardiophin1|Cardiotrophin I|Cardiotrophin-1|CardiotrophinI|CT 1|CT-1|CT1|CTF 1|Ctf1|CTF1_HUMAN |
背景资料 | Cardiotrophin-1(CT-1) is a member of the family of cytokines that includes leukemia inhibitory factor(LIF), ciliary neurotrophic factor(CNTF), oncostatin M(OSM), interleukin-6(IL6), and interleukin-11(IL11). And the CT-1 gene is mapped to 1p21-p13. The human CT-1 protein contains 201 amino acids and shares 80% amino acid identity with the 203-amino acid mouse CT-1 sequence; however, unlike the mouse protein, human CT-1 has 2 rather than 1 cys and has no N-glycosylation site. Despite lacking a signal sequence, secreted CT-1 and mouse CT-1 induce cardiac myocyte hypertrophy in cell culture and bind to both mouse and human LIFR but not to OSMR. Furthermore, A 1.7-kb CT-1 transcript was detected at high levels in heart, skeletal muscle, prostate, and ovary. Low levels were detected in lung, kidney, pancreas, thymus, testis, and small intestine, with little or no expression detected in brain, placenta, spleen, colon, and peripheral blood leukocytes. And it was also observed strong expression in fetal lung and kidney. |
显色时间 | 15-20分钟 |
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK0563-CAP | 96-well plate precoated with anti- human Cardiotrophin-1 antibody | 1 |
EK0563-ST | Lyophilized recombinant human Cardiotrophin-1 standard | 10ng/tubex2 |
EK0563-DA | Biotinylated anti- human Cardiotrophin-1 antibody | 100ul(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 100ul(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 253 | 924 | 3655 | 274 | 930 | 3410 |
Standard deviation | 12.65 | 69.3 | 288.74 | 16.16 | 70.68 | 300.08 |
CV(%) | 5% | 7.5% | 7.9% | 5.9% | 7.6% | 8.8% |
Concentration(pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
O.D. | 0.031 | 0.059 | 0.095 | 0.139 | 0.277 | 0.534 | 1.288 | 2.278 |
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