- ￥4400
- Cytoskeleton
- ACD03
- 美国
- 2025年07月14日
- Western Blot Immunocytochemistry
- mouse
- human, mouse, rat or other extracts
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- 详细信息
- 技术资料
- 供应商：
上海研卉生物科技有限公司
- 库存：
现货
- 靶点：
Cytoskeleton
- 级别：
2x200ul
- 目录编号：
2x200ul
- 克隆性：
单克隆
- 宿主：
mouse
- 适应物种：
human, mouse, rat or other extracts
- 免疫原：
Western Blot Immunocytochemistry
- 形态：
human, mouse, rat or other extracts
- 应用范围：
Western Blot Immunocytochemistry
- 浓度：
ACD03
- 规格：
2x200ul
Anti-Cdc42: mouse Mab
货号： ACD03相关产品：
Anti-Cdc42: mouse Mab sku : ACD03 Anti-Cdc42: mouse Mab
Anti-Rac1: mouse Mab sku : ARC03 Anti-Rac1: mouse Mab
Anti-RhoA: mouse IgM Mab sku : ARH05 Anti-RhoA: mouse IgM Mab
Anti-RhoA: mouse Mab sku : ARH04 Anti-RhoA: mouse Mab
介绍
Cdc42是Rho GTPase家族研究最广泛的成员之一。该家族参与广泛的细胞反应，包括细胞骨架重组、转录调节、细胞迁移、细胞转化和转移。Cdc42在大量不同类型的细胞中表达。
产品用途包括
建议将该抗体用于检测人、小鼠、大鼠或其他提取物中的Cdc42。使用该抗体测试了以下方案：
| Western Blot | Immunocytochemistry | ELISA | Immunoprecipitation |
| Yes | Yes | No | No |
Material
The anti-Cdc42 antibody (Cat. # ACD03) is a mouse monoclonal antibody that only recognizes Cdc42, not Rac1, Rac2, Rac3, RhoA, RhoB, RhoC or H-Ras. Other commercially available Cdc42 antibodies recognize one or more of these close isoforms Platelet extract (Cat. # EXT01) is included as a positive control. ACD03 is supplied as a lyophilized white powder.
Figure 1: Western blot analysis of purified protein and cell extracts using ACD03 as primary antibody. Recombinant small G-proteins and tissue extracts were separated by SDS-PAGE and transferred to PVDF membrane. ACD03 was diluted to 250 ng/ml (1:1000) in PBST plus 0.1% non-fat milk powder and Western analysis was as described in the Methods section of the datasheet. Lane 1; 25 ng Cdc42-6xHIS, Lane 2; 250 ng RhoA-6xHIS, Lane 3; 250 ng Rac1-6xHIS, Lane 4; 40 µg Swiss 3T3 cell extract, Lane 5; 40 µg bovine brain extract, Lane 6; 40 µg human platelet extract. Note: Cdc42-6xHIS runs at 25kDal whereas the native Cdc42 runs at 22kDal.
Table 1 - Reactivity of other commercially available Cdc42 antibodies
| Cdc42 |
Rac sub-family |
Rho sub-family |
Ras sub-family |
|
| Cytoskeleton, Inc. (Cat. # ACD03) |
Yes |
No |
No |
No |
| Santa Cruz (Cat. #sc-87-G) |
Yes |
Yes |
Yes |
NT |
| Cell Signalling (Cat. # 2462) |
Yes |
Yes |
NT |
NT |
NT = Not tested
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