Cycloheximide

多核糖体mRNAs是酿酒酵母中mRNA无义介导降解途径的底物

nonsense-containing transcripts of the LEU2, HIS4, PGK1, and CYH2 genes, and two wild-type mRNAs (the MAT(alpha)1 and CYH2 mRNAs), were stabilized when the translation elongation inhibitor cycloheximide was added to cellular growth media. Subsequent removal

YAC TRANSFER by KAR1

(with or without stirring). Cells are collected by centrifugation and washed twice with sterile water, and then cells are plated at about 5X10 6 cells per plate on cycloheximide medium ( 3.0 µg/ml cycloheximide) selective for the YAC transfer. YACductants

Determining Protein Half-Lives

in detail the two most frequently used methods, pulse-chase analysis and cycloheximide blocking, to determine a protein’s half-life in yeast and cultured mammalian cells.