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- 文献和实验
- 技术资料
- 保存条件:
For long storage, keep at - 80°C (or at least - 20°C). For continuous use keep at 4°C (with 0.01% Na-azide). Avoid repeated freezing and thawing.
- 克隆性:
多克隆
- 适应物种:
Mouse monoclonal, IgG1
- 供应商:
安诺伦(北京)生物科技有限公司
- 靶点:
Rat endothelial cells
- 规格:
200 µl
靶点/蛋白质:Rat endothelial cells
存储溶液:Lyophilized concentrated supernatat (no preservatives).
官网链接:https://www.swant.com/pdfs/Monoclonal_anti_endothelial-cells_288.pdf
商品关键词:Monoclonal antibody to endothelial cells.,288,Swant
简单描述:Monoclonal antibody 288 has been shown by immunohistochemistry to label the endothelial cells of the brain. The antigen recognized by this antibody is unknown.
Product: Ascites
Code No: 288
Lot No: 14.4
Reconstitution: with 200 ul bidistilled water.
官网链接: www.annoron.com
办公电话: 010-56256916
官方售后: 400-965-8633
企业QQ: 3198592576、3212874516
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文献和实验Monoclonal Antibody Expression in Mammalian Cells
are difficult for small companies and academic labs to reproduce. Transient expression in mammalian cells has recently been used very widely for monoclonal antibody expression. Cell line and expression vector engineering increased expression levels
Monoclonal Antibody Production
Positive Clones (1) The day prior to any expansion,obtain feeder cells.For this first expansion from the 200 µl cultures in 96-well plates to 500 µl cultures in 24-well plates,add 200 µl of feeder cells/well in DMEM/20% FCSHT to the 24-well plates the day
Monoclonal Antibody Production Protocol
the rinse twice. Add 100 ul of blocking solution to every well, leave 1 hr at room Temp or O.N at 4°C. PRIMARY ANTIBODY Add the antibody to be tested: Sup of cells = 25 ul, mix well by pipetting up and down (10 times).serum, ascites = 1:100 and a series
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