Freeze in small aliquots (e.g. 1 or 10 ug) and keep at -80°C. Avoid repeated freezing and thawing.
供应商:
安诺伦(北京)生物科技有限公司
规格:
100 µg
研究领域:Calretinin
靶点/蛋白质:6-His human calretinin (recombinant) produced in Escherichia Coli.
来源:Recombinant human
存储溶液:lyophilized (10 or 100 ug in 25, resp. 155 ul of: 10 mM Tris, 50 mM NaCl, 2 mM CaCl2, 1 mM EDTA, 1mM 脽-mercaptoethanol, pH 7.4).
参考文献:Schwaller B., Buchwald P., Blümcke I., Celio M.R. and Hunziker W. (1993) Characterization of a polyclonal antiserum against the purified human recombinant calcium-binding protein calretinin. Cell Calcium 14: 639-648.
简单描述:This recombinant protein has been purified by affinity chromatography on a NTA-resin This protein can be used for immunoblots, absorption experiments in immunohistochemistry, radioimmunoassay and intracellular injection. For adsorption we suggest the following procedure: - Dilute 1 ul of the antiserum 7696 (SWant) against calretinin in 5 ml of the usual buffer for immunohistochemistry (final dilution 1:5'000). - Add 1 ug of the recombinant protein to 1 ml of the diluted antibody solution and mix well. - Incubate for at least 6 hours in the cold. - Apply to tissue-sections and incubate for 3 days. - Complete the immunohistochemical reaction as usual (biotinylated second antibody, ABC-complex, DAB). As a result, the immunostaining should be strongly reduced or even completely prevented.