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- 山东省青岛市城阳区青大工业
- 2025年07月08日
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- 技术资料
- 库存：
900
- CAS号：
254109-22-3
- 保质期：
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- 供应商：
青岛捷世康生物科技有限公司
- 保存条件：
Store at -20°C
- 规格：
10 MG
参数规格
产品名称：DAF-FM diacetate；254109-22-3
规格：1mg；5mg
DAF-FM diacetate is a cell-permeable, fluorescent probe for the detection and bioimaging of nitric oxide (NO), with excitation/emission maxima of 495/515 nm. It passively diffuses across cellular membranes and, once inside cells, is then deacetylated by intracellular esterases to DAF-FM [1].
The fluorescence quantum yield of DAF-FM was ~ 0.005, but increased about 160 folds, to ~ 0.81, after reacting with NO [1]. DAF-FM fluorescence with NO is time-dependent. In a reaction mixture containing 190 nM NO or 380 nM NO, and 1 μM DAF-FM, relative fluorescence (493 nm excitation, 514 nm emission) increased rapidly during the first 10 min of incubation and reached a steady slow increase at 40 min. After 60 min, there was a minimal (~ 15%) increase in fluorescence [2].
DAF-FM diacetate exhibited several advantages over the NO probe, DAF-2 diacetate. When the pH values exceeded 5.5, the spectra of the NO adduct of DAF-FM become independent. Compared with the NO adduct of DAF-2, the combination of NO and DAF-FM was significantly more photostable. In addition, the NO detection limit of DAF-FM was ~ 3 nM, more sensitive than that of DAF-2 (~ 5 nM) [1, 3].
| Physical Appearance | A solid |
| Storage | Store at -20°C |
| M.Wt | 496.42 |
| Cas No. | 254109-22-3 |
| Formula | C25H18F2N2O7 |
| Solubility | Soluble in DMSO |
| Chemical Name | 4-amino-2',7'-difluoro-5-(methylamino)-3-oxo-3H-spiro[isobenzofuran-1,9'-xanthene]-3',6'-diyl diacetate |
| SDF | Download SDF |
| Canonical SMILES | O=C1OC2(C3=CC(F)=C(C=C3OC4=C2C=C(C(OC(C)=O)=C4)F)OC(C)=O)C5=C1C(N)=C(NC)C=C5 |
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文献和实验Investigating G Protein-Coupled Receptor Endocytosis and Trafficking by TIR-FM
, some of which occur on a rapid timescale similar to that of functional signaling itself. Here, we describe and discuss an optical imaging approach, based on evanescent field or total internal reflection-fluorescence microscopy (TIR-FM), to investigate receptor
and describe two advanced protocols that make use of the membrane binding dye FM 1-43, enabling to probe deep into the mechanisms of synaptic vesicle function and dynamics at the Drosophila third instar larval neuromuscular junction.
of the unique fluorescent FM dyes allowed the monitoring of fast synaptic vesicle exo-endocytic cycling during live imaging sessions and after photoconversion of FM dyes into an electron-dense diaminobenzidine polymer synaptic vesicle cycling can be studied
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