Human MMP-8 ELISA Kit

Product name:

Human MMP-8 ELISA Kit

Tests:

1 X 96 well plate

Sample type:

Serum, Plasma, Cell culture supernatants, Tissue lysates, Saliva

Assay type:

Sandwich

Sensitivity:

17.4 pg/mL

Range:

62.5-4000 pg/mL

Reacted Species:

Human

Tested applications:

Sandwich ELISA

Recovery:

Sample Type	Average	Range
Human serum	105%	90%-113%
cell culture supernatants	108%	84%-129%
saliva	107%	95%-121%
Tissue Lysates	85%	76%-97%

IntraAssay:

Sample	n	mean (pg/mL)	SD	CV%
1	20	416.3	10.3	2.5
2	20	638.2	16.9	2.7
3	20	2,13.3	110.7	5.5

InterAssay:

Sample	n	mean (pg/mL)	SD	CV%
1	24	348.4	9.4	2.7
2	24	594.0	20.8	3.5
3	24	2,026.5	107.3	5.3

Product overview:

KE00218 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The MMP-8 ELISA kit is to be used to detect and quantify protein levels of endogenous MMP-8. The assay recognizes human MMP-8. An antibody specific for MMP-8 has been pre-coated onto the microwells. The MMP-8 protein in samples is captured by the coated antibody after incubation.Following extensive washing, another antibody of biotinylated specific for MMP-8 is added to detect the captured MMP-8 protein. For signal development, Sterptravidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.

Storage Instructions:

All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions.

Synonyms:

CLG1, HNC, Matrix metalloproteinase 8, MMP 8, MMP8, MMP-8, Neutrophil collagenase, PMNL CL, PMNL collagenase