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发货周期:现货
博士德生物
cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).
96T
货号 | EK0535 |
---|---|
产品名称 | Human uPA/PLAU ELISA Kit |
产品描述 | For quantitative detection of human uPA in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). |
免疫原 | NSO, M1-L431 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <5pg/ml |
检测范围 | 62.5pg/ml-4000pg/ml |
基因名称 | PLAU |
基因全名 | plasminogen activator, urokinase |
Uniprot ID | P00749 |
指标别名 | ATF|BDPLT5|Plasminogen activator urinary|PLAU|QPD|u PA|U-plasminogen activator|uPA|URK|UROK_HUMAN|Urokinase-type plasminogen activator chain B |
背景资料 | Plasminogen activator,urokinase(PLAU, uPA) converts plasminogen to plasmin. Plasmin is involved in processing of amyloid precursor protein and degrades secreted and aggregated amyloid-beta, a hallmark of Alzheimer disease(AD). Urokinase has a molecular mass of about 54 kD and is composed of 2 disulfide-linked chains, A and B, of molecular masses 18 kD and 33 kD, respectively. It localized on 10q24. uPA facilitates cell migration by localizing proteolisys on the cell surface and by inducing intracellular signalling pathways. In human vascular smooth muscle cell(VSMC), uPA stimulates migration via the uPA receptor(uPAR) signalling complex containing TYK2 and phosphatidylinositol 3-kinase(PI3-K). |
显色时间 | 20-25分钟 |
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK0535-CAP | 96-well plate precoated with anti- human uPA antibody | 1 |
EK0535-ST | Lyophilized recombinant human uPA standard | 10ng/tubex2 |
EK0535-DA | Biotinylated anti- human uPA antibody | 100ul(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 100ul(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 195 | 583 | 1373 | 211 | 536 | 1493 |
Standard deviation | 8.77 | 39.06 | 74.14 | 12.87 | 44.48 | 103.01 |
CV(%) | 4.5% | 6.7% | 5.4% | 6.1% | 8.3% | 6.9% |
Concentration(pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1000 | 2000 | 4000 |
O.D. | 0.002 | 0.080 | 0.174 | 0.283 | 0.564 | 0.853 | 1.408 | 2.104 |
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