- ¥4200
- RAYBIO
- ELH-EDN1-1
- 美国
- 2025年07月14日
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 库存：
4周
- 供应商：
上海研卉生物科技有限公司
- 规格：
96T
RayBio® Human Endothelin-1 ELISA Kit for Cell Culture Supernatants, Plasma, and Serum samples.
Human Endothelin-1 ELISA
Antigen Information
- P05305
- 1906
- EDN1
- Human
Assay Format
Need more sensitivity? Check out the new IQELISA™ kit for this target. Still not enough? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa™ technology.
- Human
- Cell Culture Supernatants
- Plasma
- Serum
- Sandwich-based
- Quantitative
Product Specifications
Product Features
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
Standard Curves
Spiking & Recovery Results
Linearity Results
Application Notes
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
- Pahari, Dipak R., et al. "Effect of Minimized Perfusion Circuit on Brain Injury Markers Carnosinase and Brain?Type Fatty Binding Protein in Coronary Artery Bypass Grafting Patients."?Artificial organs?37.2 (2013): 128-135.
Species: Human
Sample type: Serum - Said, Neveen, et al. "Tumor endothelin-1 enhances metastatic colonization of the lung in mouse xenograft models of bladder cancer."?The Journal of clinical investigation?121.1 (2011): 132-147.
Species: Human
Sample type: Conditioned Media
- Pahari, Dipak R., et al. "Effect of Minimized Perfusion Circuit on Brain Injury Markers Carnosinase and Brain?Type Fatty Binding Protein in Coronary Artery Bypass Grafting Patients."?Artificial organs?37.2 (2013): 128-135.
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验人内皮素-1(Endothelin-1)ELISA试剂盒 说明书
人内皮素-1 ( Endothelin-1 )ELISA 试剂盒 ( 用于血清、血浆、细胞培养上清液和其它生物体液内 ) 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗人 Endothelin-1 单抗包被于酶标板上，标准品和样品中的 Endothelin-1与单抗结合，加入生物素化的抗人 Endothelin-1 ，形成免疫复合物连接在板上，辣根
市面上的 ELISA 试剂盒五花八门，质量良莠不齐。如何浪里淘金，选择既符合科研需求，又品质上乘的 ELISA 试剂盒呢? 教你七招，快速搞定 ELISA 试剂盒的选择！ 01 查找待测样本的蛋白浓度 通过检索 SCI 文献、Genecards、Mayocliniclabs 等，获得目标蛋白的表达部位及表达浓度参考值，比较 ELISA 试剂盒中给出的样本测值与参考值是否一致。 02 明确试剂盒的应用种属＆检测的样本类型 不同厂家验证的样本类型不同。除试剂盒特别说明外，一般情况下，不同
本文主要介绍 ELISA 试剂盒、实验样本、相关问题，帮助大家更好地开展实验 试剂盒 Q1：科研试剂盒与临床试剂盒的区别 ？ A1：临床试剂是经过权威机构认可的，如 FDA、CFDA 等；而科研试剂一般是厂家自主研制，不需要通过权威机构验证。 经营临床诊断类产品的机构需要取得《医疗器械经营企业许可证》；经营科研试剂的机构不需要此证件。 临床试剂盒一般仅用于检测人血清/血浆等较为单一的分泌性样本类型；科研试剂盒的样本检测种类、检测范围则比临床试剂盒宽广得多。但一般来说，临床试剂盒的质量
技术资料暂无技术资料 索取技术资料
