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- 详细信息
- 文献和实验
- 技术资料
- 样本:
Supernatant,Serum,Plasma
- 标记物:
NGAL
- 库存:
96T
- 供应商:
Lipocalin-2 (LCN2/NGAL) Rat ELISA Kit
- 检测方法:
elisa
- 适应物种:
大鼠
- 应用:
ERLCN2
- 检测范围:
6.25-400pg/mL
- 规格:
96T
Lipocalin-2 (LCN2/NGAL) Rat ELISA Kit
货号:ERLCN2
检测灵敏度
30 pg/mL
测定范围
32.77-8000 pg/mL
检测样本类型/体积
{Plasma=1 µL, Serum=1 µL, Supernatant=100 µL}
手工操作时间
1 hr 20 min
总时间
4 hr 45 min
均相(无需洗涤)
No
批间差异
<12%
批内差异
<10%
检测仪器
Colorimetric Microplate Reader
产品规格
96 Tests
产品成分
Pre-coated 96 well plate
Standard
Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
运输条件
Wet or Dry Ice
储存
2-8°C
蛋白名称
NGAL
蛋白别名
Alpha-2-microglobulin-related protein, Alpha-2U globulin-related protein, lipocalin 2 (oncogene 24p3), Lipocalin 24p3, Lipocalin-2, Neutrophil gelatinase-associated lipocalin, NGAL, p25, Siderocalin LCN2
蛋白家族
Proteases Peptidases & Inhibitors
均相(无需洗涤)
Rat
检测试剂盒格式
Sandwich ELISA Kit
检测抗体结合物
Biotin
标签或染料
HRP
基因别名
Lcn2, Sip24
基因ID
关于本试剂盒
The Rat Lipocalin-2 (LCN2/NGAL) ELISA quantitates Rt Lipocalin-2 in rat serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Rt Lipocalin-2.
原理方法
The Rat Lipocalin-2 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
严格验证
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
NGAL (Neutrophil gelatinase-associated lipocalin, lipocalin 2, siderocalin and neutrophil lipocalin) is a 25 kDa glycosylated single chain monomer and member of the lipocalin family of proteins that bind and transport small lipophilic molecules. NGAL is released by activated neutrophils, can form dimers and small amounts of higher oligomers, and complexes with matrix metalloproteinase 9 (MMP-9, gelatinase B). Low level expression of NGAL in a variety of epithelia may be increased in inflammation or cancers. Expression of NGAL in the kidney is dramatically increased by ischemic or nephrotoxic kidney injury.
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