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- 文献和实验
- 技术资料
- 保质期:
见产品外包装
- 供应商:
嵘崴达
- CAS号:
见产品外包装
- 英文名:
Chitinase from Streptomyces griseus
- 库存:
需确认
- 保存条件:
−20°C
- 规格:
5 units/ 25 units/ 50 units
| 规格: | 5 units | 产品价格: | ¥1220.0 |
|---|---|---|---|
| 规格: | 25 units | 产品价格: | ¥3146.0 |
| 规格: | 50 units | 产品价格: | ¥6443.0 |
Chitinase from Streptomyces griseus
lyophilized powder (essentially salt free), ≥200 units/g solid
General description
Chitinase is an extracellular complex of enzymes that degrade chitin. Chitin is a cell wall component of Fungi and exoskeketal essentials of different organisms which reshape their own chitin or digest/dissolve the chitin of other organisms (insects, fungi, yeast, and algae, and in the internal structures of other vertebrates) . Chitinases have been detected in many microorganisms and in plants. In fungi, chitinases assist in morphogenesis, to break down the inherent chitin content of fungal cell walls. Plant chitinases help in resistance to fungal attack and counteracting fungal growth, by targeting those same fungal cell walls. In bacteria, bacterial chitinases assist in utilizing chitin as a carbon source and as an energy source.Streptomyces griseus produces multiple chitinases of different molecular masses after growth induction with chitin as the carbon source.
The enzymatic hydrolysis of chitin to N-acetyl-D-glucosamine involves two consecutive enzyme reactions:
• The first reaction, chitodextrinase-chitinase, is a poly(β-(1→4)-[2-acetamido-2-deoxy-D-glucoside])- glycanohydrolase, which removes chitobiose units from chitin.
• The second activity is N-acetyl-glucosaminidasechitobiase, which cleaves the disaccharide to its monomer subunits, N-acetyl-D-glucosamine.
Application
Agriculture fields: control pathogens.
Human health care: Asthma.
Pharma: preparation of chitooligosaccharides and N-acetyl D glucosamine,
Preparation of single-cell protein
Isolation of protoplasts from fungi and yeast
Control of pathogenic fungi
Treatment of chitinous waste, mosquito control and morphogenesis
包装
5, 25, 50 units in glass bottle
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文献和实验性改变,哪怕想在一个很小的基因簇的每个基因上增加一个启动子,都需要非常多的实验步骤。相比之下,Zhao说:“事实上,我们能建立整个基因簇,这最终使我们更加灵活,因为我们能增加不同的启动子,”确保在基因簇中的每个基因都被持续激活。 研究人员修改了来自一种土壤细菌——灰色链霉菌(Streptomyces griseus)的一个隐藏的基因簇(包含6个基因)。他们在基因簇中的每个基因上增加了一个启动子,使表达增加,将基因簇插入一种近缘的细菌链霉菌(Streptomyces lividans)中
Pronase is the name given to a group of proteolytic enzymes that are produced in the culture supernatant of Streptomyces griseus K-1 (1 –3 ). Pronase is known to contain at least ten proteolytic components: five serine-type proteases, two Zn
亚胺环己酮(戊于酰亚胺环己酮) cyclohe- ximide又称放线菌酮( actidione)。从制造链霉素的放线菌灰色链丝菌 ( Streptomyces griseus)培养液中发现的抗霉性抗菌素类。用作农药。又作为真核细胞蛋白质合成抑制剂而在生物化学实验上广泛使用。对原核细胞的蛋白质生物合成无阻碍。
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