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IRF3干扰素调节因子3

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  • ¥780 - 2200
  • LMAI Bio
  • LM-2993R
  • 进口/国产
  • 2025年07月14日
  • WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 （石蜡切片需做抗原修复）
  • Rabbit
  • Human, Mouse, Rat, Dog, Pig, Cow, Horse,
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    • 文献和实验
    • 技术资料
    • 供应商：

      上海联迈生物工程有限公司

    • 库存：

      大量

    • 目录编号：

      LM-2993R

    • 克隆性：

      多克隆

    • 抗原来源：

      Rabbit

    • 保质期：

      1年

    • 抗体英文名：

      IRF3

    • 抗体名：

      干扰素调节因子3

    • 宿主：

      Rabbit

    • 适应物种：

      Human, Mouse, Rat, Dog, Pig, Cow, Horse,

    • 免疫原：

      KLH conjugated synthetic peptide derived from human IRF3:51-150/427

    • 亚型：

      IgG

    • 形态：

      Lyophilized or Liquid

    • 应用范围：

      WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 （石蜡切片需做抗原修复）

    • 浓度：

      1mg/ml

    • 保存条件：

      Store at -20 °C

    • 规格：

      50ul  100ul  200ul

    IRF3干扰素调节因子3 
    英文名称 IRF3
    中文名称 干扰素调节因子3
    别    名 Interferon regulatory factor 3; IRF-3; IRF 3; IRF3_HUMAN.  
    规格价格 50ul/780元 购买    100ul/1380元 购买    200ul/2200元 购买    大包装/询价
    说 明 书 50ul  100ul  200ul
    研究领域 肿瘤  免疫学  细胞凋亡  转录调节因子  
    抗体来源 Rabbit
    克隆类型 Polyclonal
    交叉反应 Human, Mouse, Rat, Dog, Pig, Cow, Horse, 
    产品应用 WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 （石蜡切片需做抗原修复） 
    not yet tested in other applications.
    optimal dilutions/concentrations should be determined by the end user.
    分 子 量 47kDa
    细胞定位 细胞核 细胞浆 
    性    状 Lyophilized or Liquid
    浓    度 1mg/ml
    免 疫 原 KLH conjugated synthetic peptide derived from human IRF3:51-150/427 
    亚    型 IgG
    纯化方法 affinity purified by Protein A
    储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
    保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
    PubMed PubMed
    产品介绍 background:
    This gene encodes a member of the interferon regulatory transcription factor (IRF) family. The encoded protein is found in an inactive cytoplasmic form that upon serine/threonine phosphorylation forms a complex with CREBBP. This complex translocates to the nucleus and activates the transcription of interferons alpha and beta, as well as other interferon-induced genes. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2011].

    Function:
    Key transcriptional regulator of type I interferon (IFN)-dependent immune responses and plays a critical role in the innate immune response against DNA and RNA viruses. Regulates the transcription of type I IFN genes (IFN-alpha and IFN-beta) and IFN-stimulated genes (ISG) by binding to an interferon-stimulated response element (ISRE) in their promoters. Acts as a more potent activator of the IFN-beta (IFNB) gene than the IFN-alpha (IFNA) gene and plays a critical role in both the early and late phases of the IFNA/B gene induction. Found in an inactive form in the cytoplasm of uninfected cells and following viral infection, double-stranded RNA (dsRNA), or toll-like receptor (TLR) signaling, becomes phosphorylated by IKBKE and TBK1 kinases. This induces a conformational change, leading to its dimerization and nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of the type I IFN and ISG genes. Can activate distinct gene expression programs in macrophages and can induce significant apoptosis in primary macrophages.

    Subunit:
    Monomer. Homodimer; phosphorylation-induced. Heterodimer with IRF7. Interacts with CREBBP. May interact with MAVS. Interacts with IKBKE and TBK1. Interacts with TICAM1 and TICAM2. Interacts with rotavirus A NSP1 (via C-terminus); this interaction leads to the proteasome-dependent degradation of IRF3. Interacts with RBCK1. Interacts with TRIM21. Interacts with HERC5.

    Subcellular Location:
    Cytoplasm. Nucleus. Note=Shuttles between cytoplasmic and nuclear compartments, with export being the prevailing effect. When activated, IRF3 interaction with CREBBP prevents its export to the cytoplasm.

    Tissue Specificity:
    Expressed constitutively in a variety of tissues.

    Post-translational modifications:
    Constitutively phosphorylated on many serines residues. C-terminal serine/threonine cluster is phosphorylated in response of induction by IKBKE and TBK1. Ser-385 and Ser-386 may be specifically phosphorylated in response to induction. An alternate model propose that the five serine/threonine residues between 396 and 405 are phosphorylated in response to a viral infection. Phosphorylation, and subsequent activation of IRF3 is inhibited by vaccinia virus protein E3. 
    Ubiquitinated; ubiquitination involves RBCK1 leading to proteasomal degradation. Polyubiquitinated; ubiquitination involves TRIM21 leading to proteasomal degradation. 
    ISGylated by HERC5 resulting in sustained IRF3 activation and in the inhibition of IRF3 ubiquitination by disrupting PIN1 binding. The phosphorylation state of IRF3 does not alter ISGylation.

    Similarity:
    Belongs to the IRF family.
    Contains 1 IRF tryptophan pentad repeat DNA-binding domain.

    SWISS:
    Q14653

    Gene ID:
    3661

    Database links:

    Entrez Gene: 3661 Human

    Entrez Gene: 54131 Mouse

    Omim: 603734 Human

    SwissProt: Q14653 Human

    SwissProt: P70671 Mouse

    Unigene: 289052 Human

    Unigene: 75254 Human

    Unigene: 3960 Mouse



    Important Note:
    This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

    干扰素调节因子家族是一大类对干扰素起调控作用的转录因子的统称。 一般认为干扰素调节因子(IRF)通过调节干扰素的表达而行使其抗病毒、应激、免疫调节功能。近年来，研究人员发现IRF在细胞凋亡、细胞周期、细胞分化、肿瘤发生中也起着重要的调节作用。
    产品图片 产品细节图片1
    Sample: 
    Heart (Mouse) Lysate at 40 ug
    Primary: Anti-IRF3 (bs-2993R) at 1/1000 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 47 kD
    Observed band size: 51 kD

    产品细节图片2
    Sample: 
    Heart (Mouse) Lysate at 40 ug
    Primary: Anti-IRF3 (bs-2993R) at 1/1000 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 47 kD
    Observed band size: 51 kD

    产品细节图片3
    Sample: 
    293T(Human) Cell Lysate at 30 ug
    Lung (Mouse) Lysate at 40 ug
    Lung (Rat) Lysate at 40 ug
    Primary: Anti-IRF3 (bs-2993R) at 1/2000 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 47 kD
    Observed band size: 47 kD

    产品细节图片4
    Tissue/cell: human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; 
    Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; 
    Incubation: Anti-IRF3 Polyclonal Antibody, Unconjugated(bs-2993R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

    产品细节图片5
    Tissue/cell: human colon carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; 
    Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; 
    Incubation: Anti-IRF3 Polyclonal Antibody, Unconjugated(bs-2993R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

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      -induced activation of IRF3 的研究论文，并被选为当期期刊封面。 宿主细胞通过模式识别受体（PRR）识别病原体核酸、蛋白等分子模式（PMP），激活下游信号通路，通过分子级联反应诱导 I 型干扰素（IFN-I）和 III 型干扰素（IFN-III）的产生，发挥抗病毒作用。其中，TBK1/IKKε-IRF3 信号转导在病毒诱导的天然免疫应答中发挥至关重要的作用。 ID2 是一种转录调节分子，属于 ID 蛋白家族成员，主要发挥基因的转录调控作用。ID2 在多种细胞，尤其是免疫细胞的发育

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