Human Cyclic guanosine monopho

Cyclic Guanosine 5-Monophosphate Binding to Regulatory GAF Domains of Photoreceptor Phosphodiesterase

Of the 11 families of mammalian cyclic nucleotide phosphodiesterases (PDEs), 5 contain regulatory domains capable of binding cyclic guanosine 5′-monophosphate (cGMP). The best understood of the GAF-containing PDEs is the family of rod (PDE

Localization of the Cyclic Guanosine 3,5-Monophosphate- Hydrolyzing Phosphodiesterase Type 9 in Rat Brain by Nonradioactive In Situ Hybridization

In this chapter, we describe a protocol for the localization of the cyclic guanosine 3′,5′-monophosphate-specific phosphodiesterase type 9 (PDE9) mRNA in the adult rat brain that uses digoxigenin-labeled riboprobes in a nonradioactive in situ

Extraction and Assay of Cyclic Nucleotides

Cyclic adenosine 3′,5′-monophosphate (cAMP) and cyclic guanosine 3′,5′-monophosphate (cGMP) have between them been implicated in the mediation of many physiological phenomena. Over the last 30 years, a number of techniques have been employed