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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
上海联迈生物工程有限公司
- 库存:
大量
- 目录编号:
LM-2573R
- 克隆性:
多克隆
- 抗原来源:
Rabbit
- 保质期:
1年
- 抗体英文名:
CD32
- 抗体名:
免疫球蛋白G Fc段受体Ⅱ/Fc γ RII a抗体
- 宿主:
Rabbit
- 适应物种:
Human
- 免疫原:
KLH conjugated synthetic peptide derived from human Fc gamma RII a:201-300/317
- 亚型:
IgG
- 形态:
Lyophilized or Liquid
- 应用范围:
WB=1:500-2000 ELISA=1:500-1000 Flow-Cyt=0.2μg/Test (石蜡切片需做抗原修复)
- 浓度:
1mg/ml
- 保存条件:
Store at -20 °C
- 规格:
50ul 100ul 200ul
| 英文名称 | CD32 |
| 中文名称 | 免疫球蛋白G Fc段受体Ⅱ/Fc γ RII a抗体 |
| 别 名 | CD 32; CD32 antigen; CD32A; CD32B; CD32C; CDw32; Fc gamma RII a; Fc fragment of IgG low affinity II receptor for (CD32); Fc fragment of IgG low affinity IIa receptor; Fc fragment of IgG low affinity IIa receptor for (CD32); Fc fragment of IgG low affinity IIb receptor; Fc fragment of IgG low affinity IIb receptor for (CD32) ; Fc fragment of IgG low affinity IIc receptor; Fc fragment of IgG low affinity IIc receptor for (CD32); Fc gamma receptor IIB; Fc gamma RII a; Fc gamma RII b; Fc gamma RII c; Fc gamma RIIa; Fc gamma RIIb; Fc gamma RIIc; Fc receptor, IgG, low affinity IIb; Fc-gamma RII-a; Fc-gamma RII-b; Fc-gamma RII-c; FC-gamma-RIIB; Fc-GAMMA-RIIC; Fc[g]RII; FCG 2; FCG2; FcgammaRIIC; FCGR 2; FcGR; FCGR2; FCGR2A; FCGR2A1; FCGR2B; FCGR2C; Fcgr3; Fcgr3a; FcgRII; Fcr-2; Fcr-3; FcRII a; FCRII; FcRII b; FcRII c; FcRIIC; IGFR 2; IGFR2; IgG Fc receptor II a; IgG Fc receptor II b; IgG Fc receptor II beta; IgG Fc receptor II c; IgG Fc receptor II-a; IgG Fc receptor II-b; IgG Fc receptor II-c; Immunoglobulin G Fc receptor II; Low affinity immunoglobulin gamma Fc region receptor II a; Low affinity immunoglobulin gamma Fc region receptor II; Low affinity immunoglobulin gamma Fc region receptor II b; Low affinity immunoglobulin gamma Fc region receptor II c; Low affinity immunoglobulin gamma Fc region receptor II-a; Low affinity immunoglobulin gamma Fc region receptor II-b; Low affinity immunoglobulin gamma Fc region receptor II-c; Ly-17; Ly-m20; LyM-1; Lymphocyte antigen 17; MGC23887; MGC30032. |
| 规格价格 | 50ul/780元 购买 100ul/1380元 购买 200ul/2200元 购买 大包装/询价 |
| 说 明 书 | 50ul 100ul 200ul |
| 研究领域 | 细胞生物 免疫学 细胞表面分子 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, |
| 产品应用 | WB=1:500-2000 ELISA=1:500-1000 Flow-Cyt=0.2μg/Test (石蜡切片需做抗原修复) not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 31kDa |
| 细胞定位 | 细胞膜 |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Fc gamma RII a:201-300/317 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| PubMed | PubMed |
| 产品介绍 | background: This gene encodes one member of a family of immunoglobulin Fc receptor genes found on the surface of many immune response cells. The protein encoded by this gene is a cell surface receptor found on phagocytic cells such as macrophages and neutrophils, and is involved in the process of phagocytosis and clearing of immune complexes. Alternative splicing results in multiple transcript variants. [provided by RefSeq]. Function: Binds to the Fc region of immunoglobulins gamma. Low affinity receptor. By binding to IgG it initiates cellular responses against pathogens and soluble antigens. Promotes phagocytosis of opsonized antigens. Subunit: Isoform IIB1 interacts with measles virus N protein. N protein is released in the blood following lysis of measles infected cells. This interaction presumably block inflammatory immune response. Interacts with INPP5D/SHIP1. Interacts with FGR. Interacts with LYN. Subcellular Location: Cell membrane; Single-pass type I membrane protein. Tissue Specificity: Is the most broadly distributed Fc-gamma-receptor. Expressed in monocyte, neutrophils, macrophages, basophils, eosinophils, Langerhans cells, B-cells, platelets cells and placenta (endothelial cells). Not detected in natural killer cells. Post-translational modifications: Phosphorylated by SRC-type Tyr-kinases such as LYN, BLK, FYN, HCK and SYK. Similarity: Contains 2 Ig-like C2-type (immunoglobulin-like) domains. SWISS: P12318 Gene ID: 2212 Database links: Entrez Gene: 2212 Human Entrez Gene: 2213 Human Entrez Gene: 9103 Human Entrez Gene: 116591 Rat Entrez Gene: 289211 Rat Omim: 146790 Human Omim: 604590 Human Omim: 612169 Human SwissProt: P12318 Human SwissProt: P31994 Human SwissProt: P31995 Human SwissProt: Q63203 Rat Unigene: 352642 Human Unigene: 154415 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 产品图片 | ![]() Sample: Jurkat(Human) Cell Lysate at 30 ug Primary: Anti-CD32 (bs-2573R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 31 kD Observed band size: 31 kD ![]() Sample: K562 Cell Lysate at 30 ug Primary: Anti- CD32 (bs-2573R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 31 kD Observed band size: 33 kD ![]() Blank control: U937 (blue). Primary Antibody:Rabbit Anti-CD32 antibody(bs-2573R), Dilution: 0.2μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. Protocol The cells were fixed with 2% paraformaldehyde (10 min) . Primary antibody (bs-2573R, 0.2μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed. ![]() Blank control: K562. Primary Antibody (green line): Rabbit Anti-CD32 antibody (bs-2573R-FITC) Dilution: 3μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Protocol The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20,000 events was performed. |
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文献和实验人免疫球蛋白G Fc段受体Ⅱ(FcγRⅡ/CD32)酶联免疫分析
人 免疫球蛋白G Fc 段受体Ⅱ(Fc γR Ⅱ/CD32) 酶联免疫分析 试剂盒使用说明书 本试剂仅供研究使用 目的:本试剂盒用于测定人血清,血浆及相关液体样本中免疫球蛋白G Fc 段受体Ⅱ(Fc γR Ⅱ/CD32) 的含量。 实验原理: 本试剂盒应用双抗体夹心法测定标本中 人 免疫球蛋白G Fc 段受体Ⅱ(Fc γR Ⅱ/CD32) 水平。用纯化的 人 免疫球蛋白G Fc 段受体Ⅱ(Fc γR Ⅱ/CD32
到的细胞类型表达FcR,同时反应抗体含有Fc段,都应该考虑进行Fc 阻断,来确保实验的特异性,减少非特异的产生。 那么,具体哪些细胞类型需要做Fc阻断? 首先,按FcR所结合的Ig种类不同可将其分为五类,即IgG(FcγR)、IgE(FcεR)、IgA(FcαR)、IgM(FcμR)和IgD(FcδR)。 IgG类型抗体的Fc受体是FcγR,依据和Fc片段亲和力的强弱,FcγR又可以被分为三个亚家族:FcγRI(CD64)、FcγRII(CD32)和FcγRIII(CD16)以及进一步还可以分为不同
1 - - (小鼠) TCRγ/δ限制成分? 免疫球蛋白Fc段受体 polyIgR 100 - 1 4 1q31-q42 多聚Ig受体(肝细胞上 polyIgR是HBV受体) FcγRⅠ(CD64) 70 - 3 - 1q23-q24 IgG Fc高亲和力受体 FcγRⅡ(CD32) 40 - 2 - 1q23-q24 IgG Fc低亲和力受体 FcγRⅢ(CD16) 50~70 - 2 - 1q23-q24 IgG Fc低亲和力受体 FcεRⅠα链 25
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