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- 详细信息
- 文献和实验
- 技术资料
- 英文名:
Borer deconex 22 LIQ-x
- 保质期:
2年
- 供应商:
borer
- 保存条件:
RT
- 规格:
6kg
deconex 22 PF is used in laboratories for the removal of organic
and inorganic residues and contamination from laboratory
glass and reusable laboratory material.
deconex 22 PF is suitable for:
.. chemical laboratories
.. microbiological laboratories
.. medical laboratories
for daily use in research and developement but also in pharmaceutical
industries and food industries
Properties
deconex 22 PF is:
.. free of NTA
.. alkaline
.. free of phosphate
.. free of chlorine
.. free of surfactants and
.. in powder form
deconex 22 PF is a special cleaner which has been developedfor a wide range of automated applications. The product has excellent dirt-removing properties. It even removes dried-on contamination very effectively.Due to its excellent dispersing property, it efficiently preventsa redeposition of removed contamination onto cleaned parts.Also in case of heavy contamination.The completely residue-free cleaning of laboratory glass and reusable material is an important prerequisite for the successful application of a cleaner in analytical-, synthesis- and cell culture-laboratories.deconex 22 PF is suitable for removing most different kinds of contamination, e.g.:
analysis-residues dried-in residues from synthesis like polymers or organic substances organic and inorganic salts greases oils pigments blood residues of tissue and proteins
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文献和实验各洗一次,每次3min。用PBS洗5min 加入蛋白酶K溶液(20ug/ml),于室温水解15min,去除组织蛋白。用蒸馏水洗4次,每次2min,然后按下述步骤2进行操作。 (2)冰冻组织切片预处理:将冰冻组织切片置10%中性甲醛中,于室温固定10min后,去除多余液体。用PBS洗两次,每次5min。置乙醇:乙酸(2:1)的溶液中,于-20℃处理5min,去除多余液体。用PBS洗两次,每次5min,然后按下述步骤2进行操作。 (3)培养的或从组织分离的细胞的预处理:将约 5 × 107个/ml
-T洗3次, 5~10 min/次→ECL显影或NBT/BCIP显色。 结果: 2. 荧光分光光度计分析 原理:活化的Caspase-3能够特异切割D1E2V3D4-X底物,水解D4-X肽键。根据这一特点,设计出荧光物质偶联的短肽Ac-DEVD-AMC。在共价偶联时,AMC不能被激发荧光,短肽被水解后释放出AMC,自由的AMC才能被激发发射荧光。根据释放的AMC荧光强度的大小,可以测定caspase-3的活性,从而反映Caspase-3被活化的程度。 方法
的质膜表现出对钒酸盐、寡霉素和硝酸钾不同的敏感度,分别占总 ATPase 活性的 83%、低于 1% 和 13%。IDPase 的活性占总 ATPase 活性的 4% [ 4,13,33] 。 ( 6 ) 采用不同提取方法得到的质膜疏水性蛋白通过蛋白质组学分析在质量和数量上均有差异,这表明不同的提取方法适合不同特定的质膜疏水性蛋白 [5] 。特别是与去垢剂 ( Triton X-114,单独使用 Triton X-100 和 Triton X-100 与机溶剂配合使用)相比,碱处理使 PM
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