- ¥1380 - 2200
- LMAI Bio
- LM-20790R
- 进口/国产
- 2025年07月13日
- WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
- Rabbit
- Mouse
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 供应商：
上海联迈生物工程有限公司
- 库存：
大量
- 目录编号：
LM-20790R
- 克隆性：
多克隆
- 抗原来源：
Rabbit
- 保质期：
1年
- 抗体英文名：
MDM2
- 抗体名：
双微体2癌基因抗体
- 宿主：
Rabbit
- 适应物种：
Mouse
- 免疫原：
KLH conjugated synthetic peptide derived from mouse MDM2:311-400/489
- 亚型：
IgG
- 形态：
Lyophilized or Liquid
- 应用范围：
WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
- 浓度：
1mg/ml
- 保存条件：
Store at -20 °C
- 规格：
100ul 200ul
| 英文名称 | MDM2 |
| 中文名称 | 双微体2癌基因抗体 |
| 别 名 | Double minute 2 protein; Hdm 2; HDM2; MDM 2; Mdm2 transformed 3T3 cell double minute 2 p53 binding protein (mouse) binding protein 104kDa; MDM2BP; Mouse Double Minute 2; MTBP; Murine Double Minute Chromosome 2; Oncoprotein Mdm2; p53 Binding Protein Mdm2; Ubiquitin protein ligase E3 Mdm2; MDM2_MOUSE; E3 ubiquitin-protein ligase Mdm2. |
| 规格价格 | 100ul/1380元 购买 200ul/2200元 购买 大包装/询价 |
| 说 明 书 | 100ul 200ul |
| 研究领域 | 肿瘤 细胞凋亡 表观遗传学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Mouse, |
| 产品应用 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 55kDa |
| 细胞定位 | 细胞核 细胞浆 |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from mouse MDM2:311-400/489 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| PubMed | PubMed |
| 产品介绍 | background: Inhibits TP53/p53- and TP73/p73-mediated cell cyclearrest and apoptosis by binding its transcriptional activation domain. Functions as a ubiquitin ligase E3, in the presence of E1 and E2, toward p53 and itself. Permits the nuclear export of p53 and targets it for proteasome-mediated proteolysis. Binds p53, p73, ARF(P14), ribosomal protein L5 and specifically to RNA. Can interact also with retinoblastoma protein(RB), E1A-associated protein EP300 and the E2F1 transcription factor. Forms a ternary complex with TP53/p53 and WWOX. Interacts with CDKN2AIP, MTBP, TRBG1 and USP7. Isoform Mdm2-F does not interact with TP53/p53. Interacts with PYHIN1. Interacts with, and ubiquitinates HIV-1 Tat. Belongs to the MDM2/MDM4 family. Function: E3 ubiquitin-protein ligase that mediates ubiquitination of p53/TP53, leading to its degradation by the proteasome. Inhibits p53/TP53- and p73/TP73-mediated cell cycle arrest and apoptosis by binding its transcriptional activation domain. Also acts as an ubiquitin ligase E3 toward itself and ARRB1. Permits the nuclear export of p53/TP53. Promotes proteasome-dependent ubiquitin-independent degradation of retinoblastoma RB1 protein. Inhibits DAXX-mediated apoptosis by inducing its ubiquitination and degradation. Component of the TRIM28/KAP1-MDM2-p53/TP53 complex involved in stabilizing p53/TP53. Also component of the TRIM28/KAP1-ERBB4-MDM2 complex which links growth factor and DNA damage response pathways. Subcellular Location: Nucleus, nucleoplasm. Cytoplasm. Nucleus, nucleolus. Expressed predominantly in the nucleoplasm. Interaction with ARF(P14) results in the localization of both proteins to the nucleolus. The nucleolar localization signals in both ARF(P14) and MDM2 may be necessary to allow efficient nucleolar localization of both proteins. Colocalizes with RASSF1 isoform A in the nucleus. Tissue Specificity: Ubiquitous. Isoform Mdm2-A, isoform Mdm2-B, isoform Mdm2-C, isoform Mdm2-D, isoform Mdm2-E, isoform Mdm2-F and isoform Mdm2-G are observed in a range of cancers but absent in normal tissues. Post-translational modifications: Phosphorylated in response to ionizing radiation in an ATM-dependent manner. Auto-ubiquitinated; which leads to proteasomal degradation. Deubiquitinated by USP2 leads to its accumulation and increases deubiquitinilation and degradation of p53/TP53. Deubiquitinated by USP7; leading to stabilize it. DISEASE: Note=Seems to be amplified in certain tumors (including soft tissue sarcomas, osteosarcomas and gliomas). A higher frequency of splice variants lacking p53 binding domain sequences was found in late-stage and high-grade ovarian and bladder carcinomas. Four of the splice variants show loss of p53 binding. Similarity: Belongs to the MDM2/MDM4 family. Contains 1 RanBP2-type zinc finger. Contains 1 RING-type zinc finger. Contains 1 SWIB domain. SWISS: P23804 Gene ID: 17246 Database links: Entrez Gene: 4193 Human Entrez Gene: 17246 Mouse Entrez Gene: 314856 Rat Omim: 164785 Human SwissProt: Q00987 Human SwissProt: P23804 Mouse Unigene: 484551 Human Unigene: 22670 Mouse Unigene: 447669 Mouse Unigene: 91829 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. Mdm2基因在肿瘤发生、发展的过程中起着重要作用，Mdm2蛋白过度表达与p53基因突变有重要的关联性。Mdm2基因在肿瘤发生、发展的过程中起着重要作用。MDM2广泛分布于人体正常组织中，可与p53蛋白相互作用。活化的p53导致MDM2表达，而MDM2的表达又可抑制野生型p53的转录激活作用。MDM2通常在软组织肉瘤中有过表达现象，而在膀胱癌、乳腺癌、宫颈癌、胶质细胞瘤中也有一定程度的表达，并可能与肿瘤细胞的耐药有关。 |
| 产品图片 |  Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MDM2) Polyclonal Antibody, Unconjugated (bs-20790R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. |
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验是一种抑癌蛋白，而mdm2 是一种癌蛋白，mdm2 能抑制P53转录并促进其水解，而P53能加强mdm2的转录水平和蛋白水平，这样形成一个负馈环路，所以P53在生物体内含量很低的。反建议百度 P53-MDM2，最好看那些文献综述，里面讲的比较详细 小小小白兔 非常感谢你，谢谢你。你肯定对他两的关系了解的很深，可否发给我一些实用性强的文献，想在背景知识了讲一些基础的知识，谢谢。联系：913777171@qq.com 小红赛
三句话读懂一篇 CNS：「神药」二甲双胍又出抗癌奇招；抑郁症
through reducing the RPL11-MDM2-p53 signaling。该研究揭示了一个新的肝癌癌基因——核糖体生物合成调节因子 RRS1（Ribosome biogenesis regulator 1 homolog），发生于拷贝数扩增的染色体 8q 区域，证明该基因通过抑制 RPL11-MDM2-p53 信号转导通路促进肝癌发展！愿肝癌早日被攻克，还人类以健康！图 1：来源 Science Advances2. Nature Communications：发现一种新的促长寿超长链饱和脂肪酸脂代谢和生物
【求助】P53的翻译后修饰都有哪些？ 怎样验证其修饰后与靶标启动子的结合活性的变化？
蛋白质功能的一个主要机制，p53在多个位点上可被磷酸化、顺-反异构化、乙酰化、泛素化、甲基化、糖基化等修饰，从而显示其生物学重要性。这种显示细胞或组织特异性并依赖细胞周期位置的多重修饰，是一种复杂的调节方式，随着细胞对DNA损伤、增殖、老化等产生的细胞信号的反应而发生波动。 磷酸化修饰 P53的磷酸化在多数情况下与蛋白质的稳定性有关。p53 N-末端的三个位点Ser15、Thr18、Ser20磷酸化后，使p53和其主要的负性调节因子MDM2之间的相互作用消失，而和乙
技术资料暂无技术资料 索取技术资料
