Recombinant Ferritin, Mitochon

Purification Strategy for Recombinant Formsof the Human Mitochondrial DNA Helicase

In this chapter, we present a streamlined purification for the production of near-homogeneous and high yield recombinant forms of the human mitochondrial DNA helicase. Minimizing the number of steps and the time elapsed for purification

In vitro Analysis of the Mitochondrial Preprotein Import Machinery Using Recombinant Precursor Polypeptides

of mitochondrial proteins from the cytosol through the double membrane system to their final destination. In order to elucidate the details of the translocation process, in vitro import assays have been developed that are based on the incubation of isolated active

Purification Strategies for Drosophila mtDNA Replication Proteins in Native and Recombinant Form: Mitochondrial Single-Stranded DNA-Binding Protein

Single-stranded DNA-binding proteins (SSBs) serve critical roles in DNA replication, repair, and recombination (1 ). Mitochondrial SSBs (mtSSBs) share similar physical and biochemical properties with Escherichia coli SSB (2 –