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文献和实验Cigarette Smoke Exposure as a Model of Inflammation Associated with COPD
Tubing PallFlex 25‐mm total suspended particulate (TSP) membrane filters (VWR International, Emfab #TX40H120‐WW
PriCells: Introduction of Isolation and Culture of Human Alveolar Epithelial Cells
above. The epitope of some antibodies is not yet identified, an example being the rat type I cell-specific antibody, VIIIB2. Type I cells are also reported to express carbopeptidase membrane-bound enzyme (CP-M), intercellular adhesion molecule-1 (ICAM-1), &beta
should be analyzed. Protein levels can be monitored by Western blot, immunofluorescence, ELISA or other means. (Protein can be isolated using Ambion's PARIS™ Kit.) For quantitating mRNA levels, however, qRT-PCR is again the preferred choice.An example
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