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- 库存:
1754
- 供应商:
北京博蕾德生物科技有限公司
- 规格:
1849T/96T
| 规格: | 1849T | 产品价格: | ¥1753.0 |
|---|---|---|---|
| 规格: | 96T | 产品价格: | 询价 |
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文献和实验Eccles:Protein Lysates from Tissue
Cell Lysis Buffer containing 1x Complete protease inhibitor (for embryonic mouse kidney 100uL is suitable) Crush tissue using pestle until tissue is completely pulverised taking care to keep it cold Incubate on ice for 5-10min
tube containing 100 µl sample3.If desirred,add an equal volume of 1 M NaOH to each sample and vortex (see Comments below).Add NaOH to standards as well if this option is used.4.Prepare standards containing a range of 5 to 100 micrograms protein (albumin
purification of protein complexes, in combination with in vivo biotinylation of critical transcription factors, has contributed to the analysis of the pluripotent state in mouse embryonic stem (ES) cells and made it possible to construct a protein?protein
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