相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 供应商:
上海联迈生物工程有限公司
- 库存:
大量
- 目录编号:
LM-19109R
- 克隆性:
多克隆
- 抗原来源:
Rabbit
- 保质期:
1年
- 抗体英文名:
Mus81
- 抗体名:
核酸内切酶同源蛋白MUS81抗体
- 宿主:
Rabbit
- 适应物种:
Human
- 免疫原:
KLH conjugated synthetic peptide derived from human Mus81:401-500/551
- 亚型:
IgG
- 形态:
Lyophilized or Liquid
- 应用范围:
WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 (石蜡切片需做抗原修复)
- 浓度:
1mg/ml
- 保存条件:
Store at -20 °C
- 规格:
100ul 200ul
| 英文名称 | Mus81 |
| 中文名称 | 核酸内切酶同源蛋白MUS81抗体 |
| 别 名 | Crossover junction endonuclease MUS81; FLJ21012; FLJ44872; Mus 81; MUS81; MUS81 Endonuclease Homolog; MUS81_HUMAN. |
| 规格价格 | 100ul/1380元 购买 200ul/2200元 购买 大包装/询价 |
| 说 明 书 | 100ul 200ul |
| 研究领域 | 细胞生物 免疫学 转录调节因子 表观遗传学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, |
| 产品应用 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 (石蜡切片需做抗原修复) not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 62kDa |
| 细胞定位 | 细胞核 |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Mus81:401-500/551 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| PubMed | PubMed |
| 产品介绍 | background: MUS81 encodes a helix-hairpin-helix protein involved in the response to UV- and methylation-induced DNA damage in Saccharomyces cerevisiae (1). Mus81 is important for replicational stress tolerance in both budding and fission yeast (2). Specifically, Mus81 associates with Eme1 to form an endonuclease that can process stalled replication forks before they have regressed to form a Holliday junction (2–4). Mus81 associated endonuclease resolves Holliday junctions into linear duplexes by cutting across the junction exclusively on strands of like polarity (2). In addition, Mus81 protein abundance increases in cells following exposure to agents that block DNA replication (2). Mus81 is involved in the recruitment of Cds1 to aberrant DNA structures where Cds1 modulates the activity of damage tolerance enzymes (4). The gene encoding human MUS81 maps to chromosome 11q13 and encodes a 551 amino acid protein (5). Function: Interacts with EME1 and EME2 to form a DNA structure-specific endonuclease with substrate preference for branched DNA structures with a 5'-end at the branch nick. Typical substrates include 3'-flap structures, replication forks and nicked Holliday junctions. May be required in mitosis for the processing of stalled or collapsed replication forks. Subcellular Location: Nucleus > nucleolus. Recruited to foci of DNA damage in S-phase cells. Tissue Specificity: Widely expressed. Similarity: Belongs to the XPF family. Contains 1 ERCC4 domain. SWISS: Q96NY9 Gene ID: 80198 Database links: Entrez Gene: 80198 Human Entrez Gene: 528432 Cow Entrez Gene: 71711 Mouse Entrez Gene: 293678 Rat Omim: 606591 Human SwissProt: Q1JPC1 Cow SwissProt: Q2KIT9 Cow SwissProt: Q96NY9 Human SwissProt: Q91ZJ0 Mouse SwissProt: Q4KM32 Rat Unigene: 288798 Human Unigene: 27697 Mouse Unigene: 137652 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 产品图片 | ![]() Sample: HepG2 Cell (Human) Lysate at 40 ug Hela Cell (Human) Lysate at 40 ug Primary: Anti-Mus81 (bs-19109R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 62 kD Observed band size: 62 kD |
风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验,已知空间结构的蛋白质及其同源蛋白数据库HSSP等。蛋白质回环分类数据库则是用于蛋白质结构、功能和分子设计研究的专门数据库。此外,酶、限制性内切酶、辐射杂交、氨基酸特性表、序列分析文献等,也属于二次数据库或专门数据库。 法国生物信息研究中心Infobiogen生物信息数据库目录DBCat搜集了主要400多个数据库的名称、内容、数据格式、联系地址、网址等详细信息,使用户对目前生物信息数据库有一个详尽的了解。DBCat本身也是一个具有一定数据格式的数据库。DBCat按DNA、RNA、蛋白质、基因
出来并混合在一起。象手动热启动方法一样,蜡防护层法比较烦琐,易于污染,不适用于于高通量应用。 Platinum DNA 聚合酶对于自动热启动 PCR 来说方便高效。Platinum Taq DNA 聚合酶的成分为复合有抗 Taq DNA 聚合酶单克隆抗体的重组 Taq DNA 聚合酶。此酶在常温下活性被封闭,要在 94℃- 95℃ 下加热数分钟才能够恢复酶活性。同经化学修饰用于热启动的 Taq DNA 聚合酶相比,Platinum 酶不需要在 94℃ 延时保温(10 到 15 分钟)以激活聚合
细胞凋亡事件发生时,会出现蛋白质和核酸分子结构的改变,形成新的表位。利用免疫学方法对这些表位进行鉴定,有助于判断样本中细胞凋亡事件的发生。 细胞凋亡的发生,是由于内源性核酸内切酶的激活,这种钙和镁依赖性核酸酶容易进入的核小体间区解开双链DNA,产生单/低聚核小体片段,而核小体本身由于DNA与组蛋白H1、H2A、H2B、H3和H4形成紧密复合物而不被核酸内切酶裂解。采用双抗体夹心酶免疫法,应用小鼠抗DNA和抗组蛋白的单克隆抗体,与核小体片段形成夹心结构,可特异性检测细胞溶解物中的单
技术资料暂无技术资料 索取技术资料










