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北京博蕾德生物科技有限公司
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96T
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文献和实验technical steps to recover uPA and PAI-1 protein from archived breast cancer tissues and to quantitatively determine uPA and PAI-1 protein content in tumor tissue extracts by enzyme-linked immunosorbent assay (ELISA) are described in detail. The technical
Live‐Cell Immunofluorescence Staining of Human Pluripotent Stem Cells
a standard alkaline phosphatase staining kit to reveal expression of the pluripotent stem cell marker APLP. Note that while hCD29 is expressed by all human pluripotent stem cells throughout each colony (E), this antibody fails to stain live cells
Identifying Small‐Molecule Modulators of Protein‐Protein Interactions
methods for identifying cyclic peptides that inhibit protein?protein interactions. Proteins of interest are cloned into a two?hybrid system engineered to operate in reverse, allowing the disruption of a protein complex to be coupled to cell growth. Cyclic
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