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嵘崴达
Extra Thick Blot Filter Paper, Precut, 7.5 x 10 cm
描述
Use this 7.5 x 10 cm extra thick blot absorbent filter paper in blotting applications as part of the gel, membrane, and filter paper stack, or sandwich.
Features
These blot absorbent filter papers are:
- Made of 100% cotton fiber
- Produced using ultrapure water and contain no additives
- Suitable for use with alcohol and other organic solvents
- Provide a uniform flow of buffer through the gel to the transfer membrane in a blotting sandwich
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文献和实验(HFI) Medium gel Mini gel 2 (14 x 19 cm) 2 (15.2 x 10) 2 (9 x 5.2) 2 (14 x 32cm) 2 (15.2 x 32) 2 (18.5 x 5.2) Cut a stack of paper toweling about 2mm smaller than the gel. The stack needs to be about 6cm thick. 8. Prewet nylon in DDW, then soak
) 2 (15.2 x 10) 2 (9 x 5.2)2 (14 x 32cm) 2 (15.2 x 32) 2 (18.5 x 5.2)Cut a stack of paper toweling about 2mm smaller than the gel. The stack needs to be about 6cm thick.8. Prewet nylon in DDW, then soak in alkali transfer buffer.9. Add buffer to transfer
Semi-dry Transfer Apparatus Biorad, Cat# 170-3940, or equivalent Power Supply 0 - 100 VDC (adj. current to 1 Amp) Immobilon-Ptransfer membrane 0.45 µm pore size; cut to same size as gel (Millipore, Cat# IPVH304-FO, or equivalent) Filter Paper
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