AMRESCO’s Rapid Western Blotting Kits streamline each step of Western blotting, enabling the entire procedure from the transfer step through development to be completed in less that an hour and a half. These versatile kits include all reagents except for user-supplied primary antibodies and are designed for use with standard transfer equipment. All reagents are protein-free formulations to minimize cross-reactivity and enhance antigen availability. Rapid Western Blotting Kits are offered with anti-mouse, anti-rabbit, anti-goat, or anti-rat secondary antibodies that are optimized to generate blots with low backgrounds and reduced blotches. The wet transfer and semi-dry versions of the kits, which differ only in the amount of transfer buffer included, are packaged with sufficient reagents to perform fifteen 10 cm×10 cm blots. Rapid Western Blotting Kits are compatible with both PVDF and nitrocellulose membranes and with staining with phospho-specific antibodies.