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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
上海联迈生物工程有限公司
- 库存:
大量
- 目录编号:
LM-17371R
- 克隆性:
多克隆
- 抗原来源:
Rabbit
- 保质期:
1年
- 抗体英文名:
HOXD3
- 抗体名:
同源盒蛋白D3抗体
- 宿主:
Rabbit
- 适应物种:
Human, Mouse, Rat, Chicken, Dog, Horse, Sheep,
- 免疫原:
KLH conjugated synthetic peptide derived from human HOXD3:201-300/432
- 亚型:
IgG
- 形态:
Lyophilized or Liquid
- 应用范围:
WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 (石蜡切片需做抗原修复)
- 浓度:
1mg/ml
- 保存条件:
Store at -20 °C
- 规格:
100ul 200ul
| 英文名称 | HOXD3 |
| 中文名称 | 同源盒蛋白D3抗体 |
| 别 名 | homeo box D3; Homeobox D3; Homeobox protein Hox D3; Homeobox protein Hox-4A; Homeobox protein Hox-D3; Homeobox protein HoxD3; homeodomain protein; Homeodomain protein; HOX 1D; HOX 4; Hox 4.1; Hox 4A; HOX D3; Hox-4.1 mouse homolog of; HOX1D; HOX4; Hox4.1; HOX4A; HOXD 3; HOXD3; HXD3_HUMAN; MGC10470. |
| 规格价格 | 100ul/1380元 购买 200ul/2200元 购买 大包装/询价 |
| 说 明 书 | 100ul 200ul |
| 研究领域 | 细胞生物 结合蛋白 表观遗传学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, Rat, Chicken, Dog, Horse, Sheep, |
| 产品应用 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 (石蜡切片需做抗原修复) not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 46kDa |
| 细胞定位 | 细胞核 |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human HOXD3:201-300/432 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| PubMed | PubMed |
| 产品介绍 | background: This gene belongs to the homeobox family of genes. The homeobox genes encode a highly conserved family of transcription factors that play an important role in morphogenesis in all multicellular organisms. Mammals possess four similar homeobox gene clusters, HOXA, HOXB, HOXC and HOXD, located on different chromosomes, consisting of 9 to 11 genes arranged in tandem. This gene is one of several homeobox HOXD genes located at 2q31-2q37 chromosome regions. Deletions that removed the entire HOXD gene cluster or 5' end of this cluster have been associated with severe limb and genital abnormalities. The protein encoded by this gene may play a role in the regulation of cell adhesion processes. [provided by RefSeq, Jul 2008] Function: Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis. Subcellular Location: Nucleus. Similarity: Belongs to the Antp homeobox family. Contains 1 homeobox DNA-binding domain. SWISS: P31249 Gene ID: 3232 Database links: Entrez Gene: 3232 Human Entrez Gene: 15434 Mouse Entrez Gene: 288152 Rat Omim: 142980 Human SwissProt: P31249 Human SwissProt: P09027 Mouse SwissProt: P18867 Rat Unigene: 93574 Human Unigene: 430708 Mouse Unigene: 223085 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 产品图片 | ![]() Sample: Kidney (Rat) Lysate at 40 ug Primary: Anti-HOXD3 (bs-17371R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 46 kD Observed band size: 46 kD |
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文献和实验h)即可。2. 修复大法——不仅仅是「煮一煮」微波炉修复:简单易行效果好,CST 推荐使用微波炉完成修复。合适的修复液:根据抗体说明书使用合适的修复液。用柠檬酸修复后,切片需浸泡在修复液中,自然冷却;而用 EDTA 修复后,切片可直接从修复缸中取出,直接进行下一步。注:使用不同的修复方式和不同生产商的抗体检测人肺癌组织中 EGFR 的表达。第一排为 CST 的 EGF Receptor (D38B1) XP® Rabbit mAb(#4267),EDTA 的修复方式明显优于柠檬酸盐及胃蛋白
啊!」「IHC,啥来头?」李湿兄扶了扶眼镜, 「IHC 可不是啥新鲜事儿,早在上个世纪七十年代,便已有人使用这项技术。近年来,随着『无图无真相,王道即真理』观念的强化,越来越多的研究使用这项古老且神秘的技术检测组织标本中特定蛋白的表达和分布,使科学假想与体内环境有机结合,让科学论文的质量得以升华。」以下是烧脑宝典,欲练此功,无需自宫IHC 简而言之,统共分成七步:脱蜡/复水,抗原修复,淬灭(去除内源性过氧化物酶),封闭,抗体孵育及 DAB 显色。前三步形似泡澡堂,后三步形似 Western blot。泡澡
、进行不对称的有丝分裂 D、多向祖细胞可进一步分化为定向祖细胞 E、造血祖细胞光镜下可分辨 【正确答案】 D 【答案解析】 20、 抗核抗体的性质主要是 A、IgM B、IgA C、IgG D、IgE E、1gD 【正确答案】 C 【答案解析】 抗核抗体主要是免疫球蛋白IgG。 21、 关于抗核抗体下列选项正确的是 A、是以自身抗原作为靶抗原的一组自身抗体的总称 B、抗核抗体只能与人酌细胞核起反应 C
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