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上海联迈生物工程有限公司
大量
LM-16833R
多克隆
Rabbit
1年
KRT74
细胞角蛋白74抗体
Rabbit
Human, Mouse, Dog, Sheep,
KLH conjugated synthetic peptide derived from human KRT74:401-500/529
IgG
Lyophilized or Liquid
WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 (石蜡切片需做抗原修复)
1mg/ml
Store at -20 °C
100ul 200ul
英文名称 | KRT74 |
中文名称 | 细胞角蛋白74抗体 |
别 名 | CK-74; Cytokeratin-74; K2C74_HUMAN; K5C; K6IRS4; K74; KB37; Keratin; Keratin-5c; Keratin-74; KRT5C; KRT6IRS4; Krt74; type II cytoskeletal 74; Type II inner root sheath-specific keratin-K6irs4; Type-II keratin Kb37. |
规格价格 | 100ul/1380元 购买 200ul/2200元 购买 大包装/询价 |
说 明 书 | 100ul 200ul |
研究领域 | 细胞生物 信号转导 |
抗体来源 | Rabbit |
克隆类型 | Polyclonal |
交叉反应 | Human, Mouse, Dog, Sheep, |
产品应用 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 (石蜡切片需做抗原修复) not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
分 子 量 | 58kDa |
细胞定位 | 细胞浆 |
性 状 | Lyophilized or Liquid |
浓 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human KRT74:401-500/529 |
亚 型 | IgG |
纯化方法 | affinity purified by Protein A |
储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
PubMed | PubMed |
产品介绍 | background: Keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into epithelial keratins and hair keratins. This protein belongs to a family of keratins that are specifically expressed in the inner root sheath of hair follicles.[provided by RefSeq, Jun 2009] Function: Has a role in hair formation. Specific component of keratin intermediate filaments in the inner root sheath (IRS) of the hair follicle. Subunit: Heterotetramer of two type I and two type II keratins. Tissue Specificity: Highly expressed in hair follicles from scalp. In hair, it is specifically present in the inner root sheath (IRS) of the hair follicle. Present in the IRS Huxley layer, but not in Henle layer or cuticle of the IRS. In the IRS Huxley layer, it is expressed in specialized Huxley cells, termed 'Fluegelzellen, along the area of differentiated Henle cells (at protein level). DISEASE: Defects in KRT74 are a cause of woolly hair autosomal dominant (ADWH) [MIM:194300]. A hair shaft disorder characterized by fine and tightly curled hair. Compared to normal curly hair that is observed in some populations, woolly hair grows slowly and stops growing after a few inches. Under light microscopy, woolly hair shows some structural anomalies, including trichorrexis nodosa and tapered ends. Similarity: Belongs to the intermediate filament family. SWISS: Q7RTS7 Gene ID: 121391 Database links: Entrez Gene: 121391 Human Omim: 608248 Human SwissProt: Q7RTS7 Human Unigene: 660125 Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
产品图片 | Sample: A431 Cell (Human) Lysate at 40 ug Primary: Anti-KRT74 (bs-16833R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 58 kD Observed band size: 55 kD Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (KRT74) Polyclonal Antibody, Unconjugated (bs-16833R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining. |
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