WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 (石蜡切片需做抗原修复) not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
分 子 量
58kDa
细胞定位
细胞浆
性 状
Lyophilized or Liquid
浓 度
1mg/ml
免 疫 原
KLH conjugated synthetic peptide derived from human GPT2:101-200/523
亚 型
IgG
纯化方法
affinity purified by Protein A
储 存 液
0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件
Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMed
PubMed
产品介绍
background: This gene encodes one of the two pyridoxal enzymes which catalyze a reversible transamination reaction to yield glutamate and pyruvate so these enzymes participate in amino acid metabolism and gluconeogenesis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011]
Function: Catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate.
Tissue Specificity: Expressed at high levels in muscle, adipose tissue, kidney and brain and at lower levels in the liver and breast.
Similarity: Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. Alanine aminotransferase subfamily.
SWISS: Q8TD30
Gene ID: 84706
Database links:
Entrez Gene: 84706 Human
Entrez Gene: 108682 Mouse
Entrez Gene: 307759 Rat
Omim: 138210 Human
SwissProt: Q8TD30 Human
SwissProt: Q8BGT5 Mouse
Unigene: 460693 Human
Unigene: 200423 Mouse
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
产品图片
Paraformaldehyde-fixed, paraffin embedded (rat stomach tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GPT2) Polyclonal Antibody, Unconjugated (bs-16302R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.