手机验证
上海联迈生物工程有限公司
大量
LM-1554R
多克隆
Rabbit
1年
Aquaporin 5
水通道蛋白5抗体
Rabbit
Human, Mouse, Rat, Dog, Pig, Cow, Horse, Sheep,
KLH conjugated synthetic peptide derived from mouse AQP5:201-265/265 <Cytoplasmic>
IgG
Lyophilized or Liquid
WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1μg/Test IF=1:100-500 (石蜡切片需做抗原修复)
1mg/ml
Store at -20 °C
50ul 100ul 200ul
英文名称 | Aquaporin 5 |
中文名称 | 水通道蛋白5抗体 |
别 名 | Aquaporin 5; AQP5; AQP 5; AQP-5; AQP5_HUMAN; Aquaporin-5. |
Specific References (3) | bs-1554R has been referenced in 3 publications. [IF=4.25] Lim, Jae-Yol, et al. "Establishment and Characterization of Mesenchymal Stem Cell-like Clonal Stem Cells from Mouse Salivary Glands." Tissue Engineering (2014). Mouse. PubMed:25273691 [IF=5.23] Yi, TacGhee, et al. "Single Cell Clones Purified from Human Parotid Glands Display Features of Multipotent Epitheliomesenchymal Stem Cells." Scientific Reports 6 (2016). FCM ; Human. PubMed:27824146 [IF=1.09] Wang, Wei, et al. "Expression of AQP5 and AQP8 in human colorectal carcinoma and their clinical significance." World journal of surgical oncology 10.1 (2012): 1-5. IHC-P ; Human. PubMed:23148732 |
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规格价格 | 50ul/780元 购买 100ul/1380元 购买 200ul/2200元 购买 大包装/询价 |
说 明 书 | 50ul 100ul 200ul |
研究领域 | 信号转导 通道蛋白 上皮细胞 |
抗体来源 | Rabbit |
克隆类型 | Polyclonal |
交叉反应 | Human, Mouse, Rat, Dog, Pig, Cow, Horse, Sheep, |
产品应用 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1μg/Test IF=1:100-500 (石蜡切片需做抗原修复) not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
分 子 量 | 29kDa |
细胞定位 | 细胞膜 |
性 状 | Lyophilized or Liquid |
浓 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from mouse AQP5:201-265/265 <Cytoplasmic> |
亚 型 | IgG |
纯化方法 | affinity purified by Protein A |
储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
PubMed | PubMed |
产品介绍 | background: Aquaporin 5 (AQP5) is a water channel protein. Aquaporins are a family of small integral membrane proteins related to the major intrinsic protein (MIP or AQP0). Aquaporin 5 plays a role in the generation of saliva, tears and pulmonary secretions. AQP0, AQP2, AQP5, and AQP6 are closely related and all map to 12q13. [provided by RefSeq, Jul 2008] Function: Forms a water-specific channel. Implicated in the generation of saliva, tears, and pulmonary secretions. Subcellular Location: Membrane; Multi-pass membrane protein. Similarity: Belongs to the MIP/aquaporin (TC 1.A.8) family. SWISS: Q9WTY4 Gene ID: 11830 Database links: Entrez Gene: 362 Human Entrez Gene: 11830 Mouse Entrez Gene: 25241 Rat Omim: 600442 Human SwissProt: P55064 Human SwissProt: Q9WTY4 Mouse SwissProt: P47864 Rat Unigene: 298023 Human Unigene: 45580 Mouse Unigene: 10066 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 水通道蛋白(Aquaporin,AQP)是一组与水分子通透有关的特异性细胞膜转运蛋白。其中,AQP 5在哺乳动物气管、支气管的上皮细胞表达较常见. |
产品图片 | Paraformaldehyde-fixed, paraffin embedded (rat lung tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Aquaporin 5) Polyclonal Antibody, Unconjugated (bs-1554R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining. Tissue/cell: human laryngo carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Aquaporin 5 Polyclonal Antibody, Unconjugated(bs-1554R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Blank control: Hela(blue). Primary Antibody:Rabbit Anti-Aquaporin 5 antibody(bs-1554R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. Protocol The cells were fixed with 2% paraformaldehyde (10 min). Antibody (bs-1554R, 1μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody of bs-1554R at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed. Blank control: A549(blue). Primary Antibody:Rabbit Anti-Aquaporin 5 antibody(bs-1554R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. Protocol The cells were fixed with 2% paraformaldehyde (10 min). Primary antibody (bs-1554R, 1μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed. |
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