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发货周期:现货
博士德生物
cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).
96T
货号 | EK0472 |
---|---|
产品名称 | Human Neurotrophin-3 ELISA Kit |
产品描述 | For quantitative detection of human Neurotrophin-3 in cell culture supernates and serum. |
免疫原 | NSO, Y139-T257 |
交叉反应 | There is cross-reactivates with NT-4<2.5%, and no detectable cross-reactivity with any other relevant proteins. |
敏感性 | <2pg/ml |
检测范围 | 15.6pg/ml-1000pg/ml |
基因名称 | NTF3 |
基因全名 | neurotrophin 3 |
Uniprot ID | P20783 |
指标别名 | AI316846|AI835689|HDNF|MGC129711|Nerve growth factor 2|Neurotrophic factor|Neurotrophin 3 (HDNF/NT 3)|Neurotrophin 3 precursor|Neurotrophin3|NGF 2|NGF2|NT3|NTF 3|NTF3 |
背景资料 | Neurotrophin-3(NT-3) is a new member of the nerve growth factor gene family, which plays an important role in the development and maintenance of the vertebrate nervous system. NT-3 and its receptor, called neurotrophic tyrosine kinase receptor type 3(Ntrk3; also called TrkC), are expressed early and throughout embryogenesis. NT-3 is one of five neurotrophin growth factors which shape the development of the nervous system by regulating neuronal survival and differentiation. NT-3 may be one of the central nervous system-derived factors that mediate neural crest(NC) cell proliferation in vivo. NT-3 has been mapped to human chromosome 12p and mouse chromosome 6.1 |
显色时间 | 20-25分钟 |
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK0472-CAP | 96-well plate precoated with anti- human Neurotrophin-3 antibody | 1 |
EK0472-ST | Lyophilized recombinant human Neurotrophin-3 standard | 10ng/tubex2 |
EK0472-DA | Biotinylated anti- human Neurotrophin-3 antibody | 100ul(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 100ul(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
1.96孔读板仪器。
2.自动洗板机.
3.移液器和枪头。建议使用多通道移液器/排枪。
4.Eppendorf 管。
5.额外洗板缓冲液 (neutral PBS 或 TBS。试剂盒内提供足量。如需额外缓冲液可参考如下配方)。
0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
*样品准备的相关试剂也不含在试剂盒中。Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 30 | 147 | 434 | 31 | 141 | 418 |
Standard deviation | 1.83 | 6.02 | 29.94 | 2.32 | 7.61 | 33.44 |
CV(%) | 6.1% | 4.1% | 6.9% | 7.5% | 5.4% | 8% |
Concentration(pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
O.D. | 0.014 | 0.073 | 0.148 | 0.277 | 0.561 | 0.870 | 1.686 | 2.529 |
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