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Annexin VI antibody

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  • ¥1380 - 2200
  • 康朗生物
  • kl-8997R
  • 中国/美国/德国
  • 2026年03月06日
  • WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500
  • Rabbit
  • Human, Mouse, Rat, Chicken, Dog, Pig,
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 供应商

      上海康朗生物科技有限公司

    • 库存

      大量

    • 目录编号

      kl-8997R

    • 克隆性

      多克隆

    • 抗原来源

      Rabbit

    • 保质期

      12个月

    • 抗体英文名

      Annexin VI antibody

    • 抗体名

      膜粘连蛋白6抗体

    • 宿主

      Rabbit

    • 适应物种

      Human, Mouse, Rat, Chicken, Dog, Pig,

    • 免疫原

      KLH conjugated synthetic peptide derived from human Annexin VI:601-673/673

    • 亚型

      IgG

    • 形态

      冻干粉或液体

    • 应用范围

      WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500

    • 浓度

      1mg/ml

    • 保存条件

      -20 °C

    • 规格

      100ul 200ul

    Annexin VI antibody
    中文名称 膜粘连蛋白6抗体
    别    名 Annexin VI; 67 kDa calelectrin; Annexin A6; Annexin VI p68; AnnexinA6; AnnexinVI; ANX 6; ANX A6; ANX6; ANXA 6; ANXA6; Calcium binding protein p68; Calelectrin; Calphobindin II; CalphobindinII; CBP 68; CBP68; Chromobindin 20; Chromobindin20; CPB II; CPBII; Lipocortin VI; LipocortinVI; p68; P70; Protein III; ProteinIII.  
    规格价格 100ul/1380元 购买    200ul/2200元 购买    大包装/询价
    说 明 书 100ul  200ul
    研究领域 肿瘤  细胞生物  结合蛋白  细胞膜蛋白  
    抗体来源 Rabbit
    克隆类型 Polyclonal
    交叉反应 Human, Mouse, Rat, Chicken, Dog, Pig, 
    产品应用 WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 (石蜡切片需做抗原修复) 
    not yet tested in other applications.
    optimal dilutions/concentrations should be determined by the end user.
    分 子 量 67-74kDa
    细胞定位 细胞膜 
    性    状 Lyophilized or Liquid
    浓    度 1mg/ml
    免 疫 原 KLH conjugated synthetic peptide derived from human Annexin VI:601-673/673 
    亚    型 IgG
    纯化方法 affinity purified by Protein A
    储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
    保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
    PubMed PubMed
    产品介绍 background:
    The Annexin family of calcium binding proteins is composed of at least ten mammalian genes. It is characterised by a conserved core domain which binds to phospholipids in a Ca2+ dependent manner and a unique amino terminal region which may confer binding specificity. The Annexin family has been implicated as regulators of such diverse processes as ion flux, endocytosis and exocytosis, and cellular adhesion. When overexpressed in A431 cells, Annexin VI causes a partial reversal of the transformed phenotype.

    Function:
    May associate with CD21. May regulate the release of Ca(2+) from intracellular stores.

    Tissue Specificity:
    Cytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.

    Post-translational modifications:
    Phosphorylated in response to growth factor stimulation.

    Similarity:
    Belongs to the annexin family.
    Contains 8 annexin repeats.

    SWISS:
    P08133

    Gene ID:
    309

    Database links:

    Entrez Gene: 309 Human

    Entrez Gene: 11749 Mouse

    Entrez Gene: 79125 Rat

    Omim: 114070 Human

    SwissProt: P08133 Human

    SwissProt: P14824 Mouse

    SwissProt: P48037 Rat

    Unigene: 412117 Human

    Unigene: 265347 Mouse

    Unigene: 204053 Rat



    Important Note:
    This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 
     
    产品图片 产品细节图片1
    Sample:
    Heart (Mouse) Lysate at 40 ug
    Primary: Anti-Annexin VI (bs-8997R )at 1/300 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 67/74 kD
    Observed band size: 70 kD

    产品细节图片2
    Protein: uterus(mouse) lyates at 40ug; 
    Primary: Rabbit Anti- Annexin VI (bs-8997R) at 1:300; 
    Secondary: 800CW Conjugated Goat (polyclonal) Anti-Rabbit IgG(H+L) at 1: 10000; 
    Predicted band size:67/74 kD Observed band size:67 kD
    产品细节图片3
    Paraformaldehyde-fixed, paraffin embedded (rat uterus); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Annexin VI) Polyclonal Antibody, Unconjugated (bs-8997R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

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    图标文献和实验
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    • 免疫金电镜分析

      而数目相应的增加. (3)Annexin VI是一种在老鼠肝脏细胞中对肝生理活动起重要作用的蛋白质,经过双重胶体金标记老鼠肝脏超薄切片上的抗annexin VI抗体可定量检测annexin VI,标记效率是80%.这样annexin VI成为第一个已知的参与肝细胞受体循环和转运的结构蛋白质. (4)通过胶体金免疫电镜分析兔子十二指肠上皮细胞不同位置处的胃动素和血液中的复合胺表明,与复合胺反应的胶体金集中在分泌腺的颗粒上,而与胃动素反应的胶体金在颗粒外部,表明这两种物质

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