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- Thermo
- 31100035
- 美国
- 2025年10月17日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
99
- 保质期：
已收到实际货物为准
- 供应商：
北京诺博莱德科技有限公司
- 保存条件：
已收到实际货物为准
货号： 31100035
规格
| Glutamine: | L-Glutamine |
|---|---|
| Phenol Red Indicator: | Phenol Red |
| Form: | Powder |
| HEPES Buffer: | No HEPES |
| Sodium Pyruvate Additive: | No Sodium Pyruvate |
| Glucose: | Low Glucose |
| Product Size: | 10 x 1 L |
| Shipping Condition: | Room Temperature |
描述
Medium 199 was originally developed for nutritional studies of chick embryo fibroblasts. It has broad species applicability, particularly for cultivation of non-transformed cells. Medium 199 is widely used in virology, vaccine production, and in vitro cultivation of primary explants of mouse pancreatic epithelium, and rat lens tissues. Life Technologies offers a variety of Gibco® Medium 199 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.
This M199 is modified as follows:
| With | Without |
| • L-glutamine | • HEPES |
| • Phenol Red | • Sodium bicarbonate |
The complete formulation is available.
Compared to other basal media, Medium 199 contains unique components, including adenine, adenosine, hypoxanthine, thymine, and additional vitamins. Medium 199 is available with Earle's salts for use in a CO2 incubator, or with Hank's salts for use without CO2.
In Vitro Diagnostic
Gibco® Medium 199 is manufactured in compliance with the requirements of ISO 13485: 2003 for the design, development, manufacture, and distribution of liquid and powder tissue culture media, sera, and reagents for cell culture research, industrial bioprocessing, and related markets.
Dual-Site cGMP Manufacturing
Gibco® Medium 199 is manufactured in a cGMP New York facility that is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 and ISO 9001. For supply chain continuity, Life Technologies offers a comparable Gibco® Medium 199 product made in the Scotland facility (31100-027). This second cGMP manufacturing location is also registered with the FDA as a medical device manufacturer and is certified to ISO 13485. Medium 199 contains no proteins, lipids, or growth factors. Medium 199 uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH. Powder forms of Gibco® cell culture medium require sodium bicarbonate supplementation, pH adjustment, and filtration at the time of preparation (seeprotocol for details).
在中国境内, 本产品仅限于非临床科研用途, 产品信息以中文为准。 (In China territory, the product is for Research Use only in non-clinical unit, product information in Chinese shall prevail.)
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文献和实验/F12都是应用最广泛的培养基。其他如M199、IMDM、L15培养基等也用于某些细胞的培养。 ◆ MEM是由Eagle’s基础培养基(BME)发展而来的，其中增加了组分的范围及浓度。 ◆ Dulbecco改良的BEM(DMEM )培养基是为小鼠成纤维细胞设计的，现在常用于贴壁细胞的培养。DMEM的氨基酸浓度是MEM的两倍，维生素浓度是MEM的4倍，采用双倍的HCO3-和CO2浓度起到更好的缓冲作用。最初的配方中葡萄糖含量为1000 mg/L，后来为了某些细胞的生长需要，将葡萄
相关专题 细胞培养基的配制 细胞培养专题 经典的培养基有很多种，Invitrogen (GIBCO)、Thermo Fisher(HyClone)、Sigma等公司都可以提供。其中DMEM 、RPMI 1640、MEM、DMEM /F12都是应用最广泛的培养基。其他如M199、IMDM、L15培养基等也用于某些细胞的培养。 ◆ MEM是由Eagle’s基础培养基(BME)发展而来的，其中增加了组分的范围及浓度
最广泛的培养基。其他如M199、IMDM、L15培养基等也用于某些细胞的培养。 ◆MEM是由Eagle’s基础培养基(BME)发展而来的，其中增加了组分的范围及浓度。 ◆Dulbecco改良的BEM(DMEM )培养基是为小鼠成纤维细胞设计的，现在常用于贴壁细胞的培养。DMEM的氨基酸浓度是MEM的两倍，维生素浓度是MEM的4倍，采用双倍的HCO3-和CO2浓度起到更好的缓冲作用。最初的配方中葡萄糖含量为1000 mg/L，后来为了某些细胞的生长需要，将葡萄糖含量又调整
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