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发货周期:现货
博士德生物
cell culture supernates, serum, plasma(heparin), saliva and urine.
96T
货号 | EK0465 |
---|---|
产品名称 | Human MMP-9 ELISA Kit |
产品描述 | For quantitative detection of human MMP-9 in cell culture supernates, serum, plasma(heparin), saliva and urine. |
免疫原 | NSO, A20-D707 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <5pg/ml |
检测范围 | 156pg/ml-10000pg/ml |
基因名称 | MMP9 |
基因全名 | matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase) |
Uniprot ID | P14780 |
指标别名 | 82 kDa matrix metalloproteinase-9|92 kDa gelatinase|92 kDa type IV collagenase|CLG4B|Collagenase Type 4 beta|Collagenase type IV 92 KD|EC 3.4.24.35|Gelatinase 92 KD|Gelatinase B|Gelatinase beta|GelatinaseB|GELB|Macrophage gelatinase|MANDP2|Matrix metallopeptidase 9 (gelatinase B 92kDa gelatinase 92kDa type IV collangenase)|Matrix metalloproteinase 9|MMP 9|MMP-9|MMP9|MMP9_HUMAN|Type V collagenase |
背景资料 | The 92-kD type IV collagenase is also known as 92-kD gelatinase, type V collagenase, gelatinase B, or matrix metalloproteinase-9(MMP9). The 72- and 92-kDa type IV collagenases are members of a group of secreted zinc metalloproteases. The matrix metalloproteinases(MMPs) are able to degrade the extracellular matrix and allow angiogenesis and tumor invasion. Gelatinase B, a matrix metalloproteinase that has proteolytic activity against connective tissue proteins, has been suggested to be important in the connective tissue remodeling processes associated with atherogenesis and plaque rupture. MMP-9 is predominantly expressed in neutrophils, macrophages, and mast cells, rather than in oncogene-positive neoplastic cells. The polymorphism of MMP-9 acts as a genetic factor for the development of smoking-induced pulmonary emphysema. The standard product used in this kit is recombinant human MMP-9 with the molecular mass of 95KDa. The detected MMP-9 includes zymogen and active enzyme. |
显色时间 | 25-30分钟 |
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK0465-CAP | 96-well plate precoated with anti- human MMP-9 antibody | 1 |
EK0465-ST | Lyophilized recombinant human MMP-9 standard | 10ng/tubex2 |
EK0465-DA | Biotinylated anti- human MMP-9 antibody | 100ul(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 100ul(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 237 | 1530 | 5547 | 250 | 1429 | 5731 |
Standard deviation | 18.72 | 117.81 | 438.21 | 23.5 | 135.75 | 504.32 |
CV(%) | 7.9% | 7.7% | 7.9% | 9.4% | 9.5% | 8.8% |
Concentration(pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
O.D. | 0.007 | 0.060 | 0.112 | 0.231 | 0.451 | 0.819 | 1.430 | 2.301 |
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