CYFP2; cytoplasmic FMR1 interacting protein 2; KIAA1168; p53 inducible protein; p53-inducible protein 121; PIR121; CYFP2_HUMAN.
规格价格
100ul/1380元 购买 200ul/2200元 购买 大包装/询价
说 明 书
100ul 200ul
研究领域
细胞生物 发育生物学 神经生物学 信号转导 t-淋巴细胞
抗体来源
Rabbit
克隆类型
Polyclonal
交叉反应
Human, Mouse, Rat, Chicken, Pig, Cow, Horse, Sheep,
产品应用
ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 (石蜡切片需做抗原修复) not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
分 子 量
146kDa
细胞定位
细胞核 细胞浆
性 状
Lyophilized or Liquid
浓 度
1mg/ml
免 疫 原
KLH conjugated synthetic peptide derived from human CYFIP2:251-350/1278
亚 型
IgG
纯化方法
affinity purified by Protein A
储 存 液
0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件
Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMed
PubMed
产品介绍
background: CYFIP2 is a 1,278 amino acid protein encoded by the human gene CYFIP2. CYFIP2 belongs to the CYFIP family and is involved in T-cell adhesion and p53-dependent induction of apoptosis. It interacts with FMR1, FXR1 and FXR2 and is a component of the WAVE1 complex composed of Abi-2, CYFIP2, C3orf10/HSPC300, NAP125 and WASF1/WAVE1. Upon binding to activated Rac 1, CYFIP2 causes the complex to dissociate, releasing activated WASF1. The CYFIP2 promoter contains a p53-responsive element that confers p53 binding as well as transcriptional activation of a heterologous reporter. Induced expression of CYFIP2 is sufficient for caspase activation and cellular apoptosis, reminiscent of p53 activation.
Function: CYFIP2 is involved in T cell adhesion and p53 dependent induction of apoptosis. It does not bind RNA but is up regulated significantly in CD4+ T lymphocytes from patients with multiple sclerosis. There are 2 isoforms produced by alternative splicing.
Subcellular Location: Cytoplasm perinuclear region. Cell junction, synapse, synaptosome. Treatment with leptomycin-B triggers translocation to the nucleus.
Similarity: Belongs to the CYFIP family.
SWISS: Q96F07
Gene ID: 26999
Database links:
Entrez Gene: 26999 Human
Entrez Gene: 76884 Mouse
Entrez Gene: 303073 Rat
Omim: 606323 Human
SwissProt: Q96F07 Human
SwissProt: Q5SQX6 Mouse
Unigene: 910449 Human
Unigene: 293961 Mouse
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
产品图片
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CYFIP2) Polyclonal Antibody, Unconjugated (bs-14140R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.