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LC 2.0 Sample Carousel (20 祃)
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文献和实验Protocol for FastTrack 2.0 mRNA Extraction Human Cells
Preparation of Cells Prepare or collect between 2x107 cells for each mRNA prep (will yield about 10-20μg of mRNA). If PBMCs from a whole blood sample are to be used, the sample should be prepared with Ficoll-Paque according to the manufacturer's
Immunohistochemistry: Fluorescence Protocol 2.0
. Sample Preparation and Fixation Harvest cells and wash twice in V-bottomed tubes with cold Wash Buffer by centrifugation (400 x g for 5 minutes) at 4°C to remove extracellular cytokines. Add 0.5 mL of the Fixation Buffer
reaction monitoring (MRM)-based relative quantitation of hundreds of polar metabolites in a complex sample. The method uses an aminopropyl column with acetonitrile as weak solvent and 20 mM ammonium acetate buffer (pH 9.4) as strong solvent. The method
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