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发货周期:现货
博士德生物
cell culture supernates, serum and plasma(heparin).
96T
货号 | EK0458 |
---|---|
产品名称 | Human MMP-1 ELISA Kit |
产品描述 | For quantitative detection of human MMP-1 in cell culture supernates, serum and plasma(heparin). |
免疫原 | NSO, F20-N469 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <20pg/ml |
检测范围 | 156pg/ml-10000pg/ml |
基因名称 | MMP1 |
基因全名 | matrix metallopeptidase 1 (interstitial collagenase) |
Uniprot ID | P03956 |
指标别名 | 27 kDa interstitial collagenase|CLG|CLGN|Fibroblast collagenase|Interstitial collagenase|Matrix metallopeptidase 1 (interstitial collagenase)|Matrix Metalloproteinase 1|Matrix metalloproteinase-1|MMP 1|MMP-1|MMP1|MMP1_HUMAN |
背景资料 | Matrix metalloproteinase-1(MMP-1) is also known as collagenase. Matrix metalloproteinases(MMPs) are zinc-dependent proteases that degrade extrMMP-1llular matrix proteins. The MMPs comprise a family of at least 20 proteolytic enzymes that play an essential role in tissue remodeling. Matrix metalloproteinase-1(MMP-1) plays an important role in the degradation of collagen in inflammatory diseases. MMP1(interstitial collagenase), MMP9(gelatinase B) and MMP12(macrophage elastase) are thought to be important in the development of emphysema. Smoking-induced MMP-1 might be important in the skin-ageing effects of tobacco smoking. The standard product used in this kit is human MMP-1 with the molecular mass of 51-53KDa. The detected MMP-1 includes zymogen and active enzyme. |
显色时间 | 25-30分钟 |
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK0458-CAP | 96-well plate precoated with anti- human MMP-1 antibody | 1 |
EK0458-ST | Lyophilized recombinant human MMP-1 standard | 10ng/tubex2 |
EK0458-DA | Biotinylated anti- human MMP-1 antibody | 100ul(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 100ul(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 215 | 1557 | 4510 | 235 | 1708 | 4065 |
Standard deviation | 13.97 | 118.33 | 261.58 | 19.5 | 160.55 | 308.94 |
CV(%) | 6.5% | 7.6% | 5.8% | 8.3% | 9.4% | 7.6% |
Concentration(pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
O.D. | 0.019 | 0.061 | 0.106 | 0.196 | 0.387 | 0.774 | 1.686 | 2.665 |
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