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Capto Blue high sub 25 ml samp
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文献和实验).If you assume the efficiency of the cells is between 107 and 108/µg of DNA,then you need to dilute the plasmid to a concentration of 0.1 ng/µl.Then transform 1 ml (0.1 ng)and plate both high (90% of the transformation)and low (10% of the transformation
for every experiment (Bluescript is a nice control because it will turn blue on xgal/IPTG plates).If you assume the efficiency of the cells is between 107 and 108/µg of DNA,then you need to dilute the plasmid to a concentration of 0.1 ng/µl.Then transform 1 ml (0.1 ng
HIGH RESOLUTION GENETIC FOOTPRINTING
stock solutions of Tris, EDTA, NaCl, Glycerol, and Hepes. Just before use, add DTT, reduced glutathione, and protease inhibitors where indicated, then pass through 0.2 um filter. 100 mM IPTG stock solution (MW=238.3) 595 mg IPTG 25 ml water
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