手机验证
上海康朗生物科技有限公司
大量
kl-6176R
多克隆
Rabbit
12个月
RAB8A antibody
ras癌基因家族Rab8蛋白/原癌基因c MEL抗体
Rabbit
Human, Mouse, Rat, Chicken, Cow, Sheep,
KLH conjugated synthetic peptide derived from human RAB8A:101-207/207
IgG
冻干粉或液体
WB=1:500-2000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500
1mg/ml
-20 °C
100ul 200ul
中文名称 | ras癌基因家族Rab8蛋白/原癌基因c MEL抗体 |
别 名 | MEL; Mel transforming oncogene; Oncogene c mel; Oncogene c-mel; RAB8; RAB8A; RAB8A member RAS oncogene family; RAB8A_HUMAN; Ras associated protein RAB8; Ras related protein Rab 8A; Ras-related protein Rab-8A. |
规格价格 | 100ul/1380元 购买 200ul/2200元 购买 大包装/询价 |
说 明 书 | 100ul 200ul |
研究领域 | 肿瘤 细胞生物 神经生物学 信号转导 |
抗体来源 | Rabbit |
克隆类型 | Polyclonal |
交叉反应 | Human, Mouse, Rat, Chicken, Cow, Sheep, |
产品应用 | WB=1:500-2000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 (石蜡切片需做抗原修复) not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
分 子 量 | 23kDa |
细胞定位 | 细胞浆 细胞膜 |
性 状 | Lyophilized or Liquid |
浓 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human RAB8A:101-207/207 |
亚 型 | IgG |
纯化方法 | affinity purified by Protein A |
储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
PubMed | PubMed |
产品介绍 | background: The protein encoded by this gene is a member of the RAS superfamily which are small GTP/GDP-binding proteins with an average size of 200 amino acids. The RAS-related proteins of the RAB/YPT family may play a role in the transport of proteins from the endoplasmic reticulum to the Golgi and the plasma membrane. This protein shares 97%, 96%, and 51% similarity with the dog RAB8, mouse MEL, and mouse YPT1 proteins, respectively and contains the 4 GTP/GDP-binding sites that are present in all the RAS proteins. The putative effector-binding site of this protein is similar to that of the RAB/YPT proteins. However, this protein contains a C-terminal CAAX motif that is characteristic of many RAS superfamily members but which is not found in YPT1 and the majority of RAB proteins. Although this gene was isolated as a transforming gene from a melanoma cell line, no linkage between MEL and malignant melanoma has been demonstrable. This oncogene is located 800 kb distal to MY09B on chromosome 19p13.1. Function: May be involved in vesicular trafficking and neurotransmitter release. Together with RAB11A, RAB3IP, the exocyst complex, PARD3, PRKCI, ANXA2, CDC42 and DNMBP promotes transcytosis of PODXL to the apical membrane initiation sites (AMIS), apical surface formation and lumenogenesis. Together with MYO5B and RAB11A participates in epithelial cell polarization. Subunit: Interacts with MAP4K2 and SYTL4. Interacts with SGSM1 and SGSM3 (By similarity). Interacts with OPTN. Interacts with RAB3IP. Interacts with MYO5B. Interacts with PIFO. Interacts with BIRC6/bruce. Interacts with OCRL. Subcellular Location: Cell membrane; Lipid-anchor; Cytoplasmic side (Potential). Golgi apparatus. Cytoplasm, perinuclear region. Cell projection. Note=Colocalizes with OPTN at the Golgi complex and in vesicular structures close to the plasma membrane. In the GDP-bound form, present in the perinuclear region. Shows a polarized distribution to distal regions of cell protrusions in the GTP-bound form. Colocalizes with PARD3, PRKCI, EXOC5, OCLN, PODXL and RAB11A in apical membrane initiation sites (AMIS) during the generation of apical surface and luminogenesis. Similarity: Belongs to the small GTPase superfamily. Rab family. SWISS: P61006 Gene ID: 4218 Database links: Entrez Gene: 100125881 Cow Entrez Gene: 403773 Dog Entrez Gene: 4218 Human Entrez Gene: 17274 Mouse Entrez Gene: 117103 Rat Omim: 165040 Human SwissProt: Q5F470 Chicken SwissProt: A4FV54 Cow SwissProt: P61007 Dog SwissProt: P61006 Human SwissProt: P55258 Mouse SwissProt: P35280 Rat Unigene: 642874 Human Unigene: 709846 Human Unigene: 162811 Mouse Unigene: 16064 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
产品图片 | Sample: NIH/3T3(Mouse) Cell Lysate at 30 ug Primary: Anti-RAB8A (bs-6176R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 23 kD Observed band size: 23 kD Sample: HepG2(Human) Cell Lysate at 30 ug Hela(Human) Cell Lysate at 30 ug Primary: Anti-RAB8A (bs-6176R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 23 kD Observed band size: 23 kD Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (RAB8A) Polyclonal Antibody, Unconjugated (bs-6176R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining. |
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