手机验证
上海康朗生物科技有限公司
大量
kl-6003R
多克隆
Rabbit
12个月
DAL1/EPB41L3 antibody
细胞骨架4.1蛋白家族DAL1抗体
Rabbit
Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit, Sheep,
KLH conjugated synthetic peptide derived from human DAL1/EPB41L3:131-230/1087
IgG
冻干粉或液体
WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500
1mg/ml
-20 °C
50ul 100ul 200ul
中文名称 | 细胞骨架4.1蛋白家族DAL1抗体 |
别 名 | Differentially expressed in adenocarcinoma of the lung protein 1; 4.1B; Band 4.1 like protein 3; DAL 1; DAL1; EPB41L3; Erythrocyte membrane protein band 4.1 like 3; HGNC:3380; KIAA0987; E41L3_HUMAN. |
规格价格 | 50ul/780元 购买 100ul/1380元 购买 200ul/2200元 购买 大包装/询价 |
说 明 书 | 50ul 100ul 200ul |
研究领域 | 肿瘤 细胞生物 免疫学 神经生物学 细胞骨架 细胞膜蛋白 |
抗体来源 | Rabbit |
克隆类型 | Polyclonal |
交叉反应 | Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit, Sheep, |
产品应用 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 (石蜡切片需做抗原修复) not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
分 子 量 | 120kDa |
细胞定位 | 细胞浆 细胞膜 |
性 状 | Lyophilized or Liquid |
浓 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human DAL1/EPB41L3:131-230/1087 |
亚 型 | IgG |
纯化方法 | affinity purified by Protein A |
储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
PubMed | PubMed |
产品介绍 | background: EPB41L3 is an actin-binding protein originally identified as a molecule whose expression is down-regulated in lung adenocarcinoma. EPB41L3 is a member of the protein 4.1 superfamily, which encompasses structural proteins that play important roles in membrane processes via interactions with actin, spectrin, and the cytoplasmic domains of integral membrane proteins. The gene for EPB41L3 is thought to function as a tumor suppressor and localizes within chromosomal region 18p11.3, which is affected by loss of heterozygosity (LOH) in various adult tumors. Function: Critical growth regulator in the pathogenesis of meningiomas. Subunit: Interacts with CADM1. Subcellular Location: Cytoplasm, cytoskeleton Tissue Specificity: Expressed at high levels in brain, with lower levels in kidney, intestine, and testis. Similarity: ontains 1 FERM domain. SWISS: Q9Y2J2 Gene ID: 23136 Database links: Entrez Gene: 23136 Human Entrez Gene: 13823 Mouse Entrez Gene: 116724 Rat Omim: 605331 Human SwissProt: Q9Y2J2 Human SwissProt: Q9WV92 Mouse Unigene: 213394 Human Unigene: 131135 Mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
产品图片 | Sample: Brain (Mouse) Lysate at 40 ug Brain (Rat) Lysate at 40 ug U-87MG (Human) CellLysate at 30 ug HepG2 (Human) CellLysate at 30 ug Primary: Anti-DAL1/EPB41L3 (bs-6003R) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 120 kD Observed band size: 130-140 kD Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (DAL1(EPB41L3)) Polyclonal Antibody, Unconjugated (bs-6003R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (DAL1(EPB41L3)) Polyclonal Antibody, Unconjugated (bs-6003R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. |
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