大家都在搜
手机验证
上海康朗生物科技有限公司
大量
kl-5853R
多克隆
Rabbit
12个月
ADAM23 antibody
去整合素样金属蛋白酶23抗体
Rabbit
Human, Mouse, Rat, Pig, Cow, Horse, Rabbit,
KLH conjugated synthetic peptide derived from human ADAM23:551-650/832 <Extracellular>
IgG
冻干粉或液体
WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=0.2ug/Test IF=1:100-500
1mg/ml
-20 °C
50ul 100ul 200ul
中文名称 | 去整合素样金属蛋白酶23抗体 |
别 名 | A disintegrin and metalloproteinase domain 23; MDC 3; Metalloproteinase like disintegrin like and cysteine rich protein 3. |
规格价格 | 50ul/780元 购买 100ul/1380元 购买 200ul/2200元 购买 大包装/询价 |
说 明 书 | 50ul 100ul 200ul |
研究领域 | 肿瘤 细胞生物 免疫学 信号转导 |
抗体来源 | Rabbit |
克隆类型 | Polyclonal |
交叉反应 | Human, Mouse, Rat, Pig, Cow, Horse, Rabbit, |
产品应用 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=0.2ug/Test IF=1:100-500 (石蜡切片需做抗原修复) not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
分 子 量 | 75-95kDa |
细胞定位 | 细胞膜 分泌型蛋白 |
性 状 | Lyophilized or Liquid |
浓 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human ADAM23:551-650/832 <Extracellular> |
亚 型 | IgG |
纯化方法 | affinity purified by Protein A |
储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
PubMed | PubMed |
产品介绍 | background: ADAM23 is a non-catalytic metalloprotease-like protein. It is highly expressed in the brain, primarily in the amygdala, caudate nucleus, hypothalamus, thalamus, cerebral cortex and occipital pole, and weakly expressed in the heart. 3 isoforms are produced by alternative splicing, alpha, beta and gamma. ADAM23 may play a role in cell-cell and cell-matrix interactions. Subunit: Can bind to LGI1 and LGI4 (By similarity). Ligand for integrin alpha-V/beta-3. Subcellular Location: Cell membrane; Single-pass type I membrane protein. Isoform Gamma: Secreted. Tissue Specificity: Highly expressed in the brain and weakly expressed in the heart. In the brain, expressed prominently in the amygdala, caudate nucleus, hypothalamus, thalamus, cerebral cortex and occipital pole. Similarity: Contains 1 disintegrin domain. Contains 1 EGF-like domain. Contains 1 peptidase M12B domain. SWISS: O75077 Gene ID: 8745 Database links: Entrez Gene: 8745 Human Entrez Gene: 23792 Mouse Entrez Gene: 301460 Rat Omim: 603710 Human SwissProt: O75077 Human SwissProt: Q9R1V7 Mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 细胞外基质蛋白 |
产品图片 | Sample: Raji Cell (Human) Lysate at 40 ug Primary: Anti-ADAM23 (bs-5853R) at 1/300 dilution Secondary: HRP conjugated Goat-Anti-rabbit IgG (bs-0295G-HRP) at 1/5000 dilution Predicted band size: 75-95kD Observed band size: 75/35 kD Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ADAM23) Polyclonal Antibody, Unconjugated (bs-5853R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining. |
风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。