Human, Mouse, Rat, Chicken, Pig, Horse, Rabbit, Sheep,
产品应用
WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 (石蜡切片需做抗原修复) not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
分 子 量
131kDa
细胞定位
细胞浆 细胞膜
性 状
Lyophilized or Liquid
浓 度
1mg/ml
免 疫 原
KLH conjugated synthetic peptide derived from human Golgi Complex:321-420/1179 <Extracellular>
亚 型
IgG
纯化方法
affinity purified by Protein A
储 存 液
0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件
Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMed
PubMed
产品介绍
background: Binds fibroblast growth factor and E-selectin (cell-adhesion lectin on endothelial cells mediating the binding of neutrophils).
Function: Binds fibroblast growth factor and E-selectin (cell-adhesion lectin on endothelial cells mediating the binding of neutrophils).
Subcellular Location: Golgi apparatus membrane.
Tissue Specificity: Widely expressed. Highest levels in pancreas, skeletal muscle, placenta, heart, testis and ovary. Also found in the kidney, liver, lung and brain.
Post-translational modifications: Fucosylation is essential for binding to E-selectin. N-glycosylated. Contains sialic acid residues.
DISEASE: Expressed both in adult and fetal tissues.
Similarity: Contains 16 Cys-rich GLG1 repeats.
SWISS: Q92896
Gene ID: 2734
Database links:
Entrez Gene: 396492 Chicken
Entrez Gene: 2734 Human
Entrez Gene: 20340 Mouse
Omim: 600753 Human
SwissProt: Q02391 Chicken
SwissProt: Q92896 Human
SwissProt: Q61543 Mouse
Unigene: 109731 Human
Unigene: 276271 Mouse
Unigene: 440619 Mouse
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
产品图片
Paraformaldehyde-fixed, paraffin embedded (Mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Golgi Complex) Polyclonal Antibody, Unconjugated (bs-13485R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.