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发货周期:现货
博士德生物
cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).
Rat
96T
货号 | EK0455 |
---|---|
产品名称 | Rat CCL20/MIP-3 alpha ELISA Kit |
产品描述 | For quantitative detection of rat MIP-3 alpha in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). |
免疫原 | E.coli, S27-M96 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <1pg/ml |
检测范围 | 31.2pg/ml-2000pg/ml |
基因名称 | CCL20 |
基因全名 | chemokine (C-C motif) ligand 20 |
Uniprot ID | CCL20: P97884 |
指标别名 | Beta chemokine exodus 1|Beta-chemokine exodus-1|C C motif chemokine 20|CC chemokine LARC|Ccl20|CCL20(2-70)|CCL20_HUMAN|Chemokine (C C motif) ligand 20|CKb4|Exodus 1|LARC|Liver and activation regulated chemokine|Liver and activation-regulated chemokine|Macrophage inflammatory protein 3 alpha|MIP 3 alpha|MIP 3a|MIP-3-alpha|MIP3 alpha|MIP3A|MIP3alpha|SCYA20|Small inducible cytokine A20|Small inducible cytokine subfamily A (Cys Cys) member 20|Small-inducible cytokine A20|ST38 |
背景资料 | Macrophage Inflammatory Protein 3alpha(MIP3alpha), also called Chemokine, cc motif, ligand 20(CCL20). The MIP-3alpha/CCL20 gene was cloned and sequenced, revealing a four exon, three intron structure, and was localized by FISH analysis to 2q35-q36. MIP3alpha is predominantly expressed in lymph nodes, appendix, PBL, fetal liver, fetal lung and several cell lines. MIP3alpha/CCL20 and its receptor CCR6 are markedly up-regulated in psoriasis, and they may play a role in the recruitment of T cells to lesional psoriatic skin. And Alanine MIP-3alpha and Serine MIP-3alpha, the two forms of MIP3alpha, that differ by one amino acid at the predicted signal peptide cleavage site. Both of them were chemically synthesized and tested for biological activity. And both flu antigen plus IL-2-activated CD4(+) and CD8(+) T lymphoblasts and cord blood-derived dendritic cells responded to Ser and Ala MIP-3alpha. |
显色时间 | 25-30分钟 |
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK0455-CAP | 96-well plate precoated with anti- rat MIP-3 alpha antibody | 1 |
EK0455-ST | Lyophilized recombinant rat MIP-3 alpha standard | 10ng/tubex2 |
EK0455-DA | Biotinylated anti- rat MIP-3 alpha antibody | 100ul(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 100ul(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 93 | 283 | 782 | 90 | 270 | 852 |
Standard deviation | 4.83 | 14.15 | 43.79 | 5.31 | 15.66 | 56.23 |
CV(%) | 5.2% | 5% | 5.6% | 5.9% | 5.8% | 6.6% |
Concentration(pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
O.D. | 0.027 | 0.126 | 0.145 | 0.240 | 0.313 | 0.497 | 1.188 | 2.014 |
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