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上海康朗生物科技有限公司
大量
kl-1103R
多克隆
Rabbit
12个月
PD-L1 antibody
程序性死亡配体1抗体
Rabbit
Mouse, Rat,
KLH conjugated synthetic peptide derived from mouse B7-H1:201-290/290
IgG
冻干粉或液体
WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500
1mg/ml
-20 °C
50ul 100ul 200ul
中文名称 | 程序性死亡配体1抗体 |
别 名 | CD274; B7 H; B7 H1; B7 homolog 1; B7-H1; B7H; B7H1; CD 274; CD274 antigen; CD274 molecule; MGC142294; MGC142296; OTTHUMP00000021029; PD L1; PD1L1_HUMAN; PD1L1_Mouse; PDCD1 ligand 1; PDCD1L1; PDCD1LG1; PDL 1; PDL1; Programmed cell death 1 ligand 1; Programmed death ligand 1; RGD1566211. |
规格价格 | 50ul/780元 购买 100ul/1380元 购买 200ul/2200元 购买 大包装/询价 |
说 明 书 | 50ul 100ul 200ul |
研究领域 | 肿瘤 细胞生物 信号转导 细胞凋亡 |
抗体来源 | Rabbit |
克隆类型 | Polyclonal |
交叉反应 | Mouse, Rat, |
产品应用 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 (石蜡切片需做抗原修复) not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
分 子 量 | 32kDa |
细胞定位 | 细胞膜 |
性 状 | Lyophilized or Liquid |
浓 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from mouse B7-H1:201-290/290 |
亚 型 | IgG |
纯化方法 | affinity purified by Protein A |
储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
PubMed | PubMed |
产品介绍 | background: Programmed cell death 1 ligand 1(Programmed death ligand 1;PD-L1; PDCD1 ligand 1; B7 homolog 1; B7-H1; CD274) involved in the costimulatory signal, essential for T lymphocyte proliferation and production of IL10 and IFNG, in an IL2-dependent and a PDCD1-independent manner. Interaction with PDCD1 inhibit T-cell proliferation and cytokine production. Function: Involved in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation and cytokine production. Subunit: Interacts with PDCD1. Subcellular Location: Isoform 1: Cell membrane; Single-pass type I membrane protein. Isoform 2: Endomembrane system; Single-pass type I membrane protein. Tissue Specificity: Highly expressed in the heart, skeletal muscle, placenta and lung. Weakly expressed in the thymus, spleen, kidney and liver. Expressed on activated T- and B-cells, dendritic cells, keratinocytes and monocytes. Similarity: Belongs to the immunoglobulin superfamily. BTN/MOG family. Contains 1 Ig-like C2-type (immunoglobulin-like) domain. Contains 1 Ig-like V-type (immunoglobulin-like) domain. SWISS: Q9EP73 Gene ID: 60533 Database links: Entrez Gene: 29126 Human Entrez Gene: 60533 Mouse Entrez Gene: 499342 Rat Omim: 605402 Human SwissProt: Q9NZQ7 Human SwissProt: Q9EP73 Mouse Unigene: 521989 Human Unigene: 245363 Mouse Unigene: 228198 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 程序性死亡配体1(B7-H1)可以促进上皮细胞的恶性转化,保护表皮细胞免于失巢凋亡,在肿瘤的发生,进展和转归中发挥重要作用. 目前对CD274信号通路的进一步的研究必将为自身免疫性疾病、病毒感染性疾病和器官移植后排斥反应中T细胞介导的免疫应答调控提供了新的途径.B7-H1在癌症、类风湿、HIV感染等疾病中发现B7-H1有负调节作用。对B7-H1路径进行调控有助于自身免疫病及恶性肿瘤的治疗。 |
产品图片 | Sample: Spleen (Mouse) Lysate at 40 ug Primary: Anti- CD274 (bs-1103R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/10000 dilution Predicted band size: 32 kD Observed band size: 48 kD Sample: Lymph node (Mouse) Lysate at 40 ug Primary: Anti- CD274 (bs-1103R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/10000 dilution Predicted band size: 32 kD Observed band size: 48 kD Tissue/cell: Mouse placenta tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37∩ for 20 min; Incubation: Anti-CD274 Polyclonal Antibody, Unconjugated(bs-1103R) 1:500, overnight at 4∑C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining |
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