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- BioporTo
- DIA HE1A-01
- 2025年07月14日
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- 形态：
IgG
- 保存条件：
-20
- 克隆性：
多克隆
- 规格：
100µl
Product nameAnti-Leptin
Description：Mouse monoclonal antibody, biotinylated
Specificity：ABS 022-11 binds human leptin.
Immunogen：Recombinant human leptin
Application：ELISA
Species reactivity (positive)：Human
Species reactivity (negative)：Not determined
Subclass：IgG1/k
Gene ID：3952
Clone number：11
Epitope：The epitope of ABS 022-11 differs from the epitope of ABS 022-03 but overlaps with the epitope of ABS 022-06
Content
100 µL, 1 mg/mL +/- 15%. See Certificate of Analysis for details.
Purification
Biotinylated
Form
Liquid：Solvent
0.01 M phosphate buffer, pH 7.4, with 0.14 M NaCl and 15 mM sodium azide
Storage
4-8ºC without exposure to light. No precautions necessary during handling.
Target
Leptin, also called obesity factor, is a protein produced by adipose tissue, which has a regulatory influence on food intake, energy metabolism and reproduction. Leptin may also play a role in immune and inflammatory responses and hematopoiesis.
Application 1
ELISA
In sandwich ELISA, bioinylated ABS 022-11 works as a detection antibody with ABS 022-03 as capture antibody.
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文献和实验as the human leukocyte antigen (HLA) locus, is a cluster of genes on human chromosome 6 that has a pivotal role in the immune response. Every person receives one set of MHC genes from each parent, and both sets of genes are expressed on the individual's cells
Detection of Known Allergen-Specific IgE Antibodies by Immunological Methods
are incubated with the respective diluted serum sample (approximately 25 μL serum required) and bound IgE antibodies are detected with a secondary horseradish peroxidase (HRP) labelled anti-human-IgE antibody via chemiluminescence. The measurement
Blocking of unwanted non-specific staining
is not. Blocking of endogenous peroxidase is done by preincubating the slides in 3 H2O2 1 Sodium Azide PBS 30 minutes several washes This mixture is more effective than 1.5 H2O2 in absolute methanol Ref. Methanol incubation is also a fixation step
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