- ¥1380 - 2200
- 康朗生物
- kl-41107R
- 中国/美国/德国
- 2025年12月18日
- WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500
- Rabbit
- Human, Mouse, Rat,
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
上海康朗生物科技有限公司
- 库存：
大量
- 目录编号：
kl-41107R
- 克隆性：
多克隆
- 抗原来源：
Rabbit
- 保质期：
12个月
- 抗体英文名：
Myoglobin antibody
- 抗体名：
肌红蛋白抗体
- 宿主：
Rabbit
- 适应物种：
Human, Mouse, Rat,
- 免疫原：
Purified Human Myoglobin (Natural Human Myoglobin protein):
- 亚型：
IgG
- 形态：
冻干粉或液体
- 应用范围：
WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500
- 浓度：
1mg/ml
- 保存条件：
-20 °C
- 规格：
100ul 200ul
| 中文名称 | 肌红蛋白抗体 |
| 别 名 | MYO; MB; MGC13548; PVALB; MYG_HUMAN. |
| 规格价格 | 100ul/1380元 购买 200ul/2200元 购买 大包装/询价 |
| 说 明 书 | 100ul 200ul |
| 研究领域 | 肿瘤 心血管 细胞生物 免疫学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, Rat, |
| 产品应用 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 17kDa |
| 细胞定位 | 细胞浆 |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | Purified Human Myoglobin (Natural Human Myoglobin protein): |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| PubMed | PubMed |
| 产品介绍 | background: Myoglobin is a small heme containing protein responsible for the oxygen deposition in muscle tissues. Only one form of myoglobin is expressed in cardiac and skeletal muscles. Myoglobin is known as a marker of myocardial damage and it has been used for more than three decades. Nowadays it still is very commonly used in clinical practice as an early marker of AMI. It appears in patient's blood 1 to 3 hours after onset of the symptoms, reaching peak level within 8 to 12 hours. Myoglobin is not so cardiac specific as cTnI or cTnT. Because of high myoglobin concentration in skeletal muscle tissue, even minor skeletal muscle injury results in the significant increase of myoglobin concentration in blood. Thus myoglobin is used together with cTnI or cTnT in clinical practise for better specificity in AMI diagnosis. Function: Serves as a reserve supply of oxygen and facilitates the movement of oxygen within muscles. Similarity: Belongs to the globin family. SWISS: P02144 Gene ID: 4151 Database links: Entrez Gene: 4151 Human Entrez Gene: 17189 Mouse Entrez Gene: 59108 Rat Omim: 160000 Human SwissProt: P02144 Human SwissProt: P04247 Mouse SwissProt: Q9QZ76 Rat Unigene: 517586 Human Unigene: 404074 Mouse Unigene: 40511 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 肌红蛋白存在于肌肉中，心肌中含量特别丰富，，有较高的组织特异性，是横纹肌的特异性标记物，但对肿瘤的敏感性较差。主要用于横纹肌肿瘤的诊断与鉴别诊断。少数恶性中胚叶混合瘤、睾丸畸胎瘤、肾母细胞瘤亦可见阳性表达。 |
| 产品图片 |  Sample: Human Myoglobin Protein at 100 ng Muscle (Mouse) Lysate at 30 ug Muscle (Rat) Lysate at 20 ug Primary: Anti-Myoglobin (bs-41107R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 17 kD Observed band size: 14 kD  Paraformaldehyde-fixed, paraffin embedded (Rat skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Myoglobin) Polyclonal Antibody, Unconjugated (bs-41107R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.  Paraformaldehyde-fixed, paraffin embedded (Human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Myoglobin) Polyclonal Antibody, Unconjugated (bs-41107R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining. |
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文献和实验Myoglobin represents the stores of oxygen in muscle tissues. Because of its relatively small mol wt, myoglobin is often used in electrophoretic techniques as a mol wt marker, and also as a test for separation efficiency in capillary
与血红蛋白相似的血红素蛋白质。存在于肌肉细胞内，可作为结晶体分离出来。分子量1万 7千，每 1分子含 1个血红素，与血红素作为四聚体活动不同，肌红蛋白是单体。人的肌红蛋白每 1分子含 1分子半胱氨酸，而马和抹香鲸的肌红蛋白则完全不含半胱氨酸。 J． C． Kendrew等（ 1958— 1963）通过 X线衍射，已弄清抹香鲸肌红蛋白结晶的立体结构。蛋白质部分的珠蛋白是由 153个氨基酸残基组成的一条多肽链，铁血红素一方面和此链中的一个组氨酸，另一方面通过 H2 O和另一个组氨酸形成配位
上海西唐生物科技有限公司 021-55229872, 65333639 www.westang.com 猪肌红蛋白 ( Myoglobin )ELISA 试剂盒 ( 用于血清、血浆、细胞培养上清液和其它生物体液内 ) 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗猪 Myoglobin 单抗包被于酶标板上，标准品和样品中的 Myoglobin与单抗结合
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