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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
上海康朗生物科技有限公司
- 库存:
大量
- 目录编号:
kl-23008R
- 克隆性:
多克隆
- 抗原来源:
Rabbit
- 保质期:
12个月
- 抗体英文名:
IL-12 antibody
- 抗体名:
白介素12抗体
- 宿主:
Rabbit
- 适应物种:
Mouse, Rat,
- 免疫原:
KLH conjugated synthetic peptide derived from mouse IL-12:21-100/215
- 亚型:
IgG
- 形态:
冻干粉或液体
- 应用范围:
WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=3ug/Test ICC=1:100-500 IF=1:100-500
- 浓度:
1mg/ml
- 保存条件:
-20 °C
- 规格:
100ul 200ul
| 中文名称 | 白介素12抗体 |
| 别 名 | IL12; IL-12; CLMF p35; CLMF1; CTL maturation factor (TcMF); Cytotoxic lymphocyte maturation factor 1; Cytotoxic lymphocyte maturation factor 35 kDa subunit; IL 12 subunit p35; IL12A; Interleukin 12 alpha chain; Interleukin 12 p35; Interleukin 12A; Natural killer cell stimulatory factor 1; CLMF; CLMF p35; Cytotoxic lymphocyte maturation factor 35 kDa subunit; IL-12 subunit p35; IL-12A; IL12 subunit p35; IL12A; IL12A_HUMAN; Interleukin 12 p35; Interleukin 12A; Interleukin-12 subunit alpha; NFSK; NK cell stimulatory factor chain 1; NKSF1. NK cell stimulatory factor chain 1; NKSF1; p35; IL12A_MOUSE. |
| 规格价格 | 100ul/1380元 购买 200ul/2200元 购买 大包装/询价 |
| 说 明 书 | 100ul 200ul |
| 研究领域 | 肿瘤 细胞生物 免疫学 生长因子和激素 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Mouse, Rat, |
| 产品应用 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=3ug/Test ICC=1:100-500 IF=1:100-500 (石蜡切片需做抗原修复) not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 22kDa |
| 细胞定位 | 分泌型蛋白 |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from mouse IL-12:21-100/215 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| PubMed | PubMed |
| 产品介绍 | background: IL-12 protein is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract. Function: Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated Killer cells, and stimulate the production of IFN-gamma by resting PBMC. Subunit: Heterodimer with IL12B; disulfide-linked. The heterodimer is known as interleukin IL-12. Subcellular Location: Secreted. Similarity: Belongs to the IL-6 superfamily. SWISS: P43431 Gene ID: 16159 Database links: Entrez Gene: 16159 Mouse Entrez Gene: 84405 Rat SwissProt: P43431 Mouse SwissProt: Q9R103 Rat Unigene: 103783 Mouse Unigene: 207199 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 产品图片 | ![]() Blank control (blue line): Mouse spleen (fixed with 70% ice-cold methanol overnight at 4℃). Primary Antibody (green line): Rabbit Anti- IL-12 antibody (bs-23008R),ilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC,Dilution: 1μg /test. |
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人白介素-12 p40(IL-12 p40)ELISA试剂盒 说明书
上海西唐生物科技有限公司 021-55229872, 65333639 www.westang.com 人白介素-12 p40 (IL-12 p40)ELISA 试剂盒 ( 用于血清、血浆、细胞培养上清液和其它生物体液内 ) 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗人 IL-12 p40 单抗包被于酶标板上,标准品和样品中的 IL-12 p40与单抗
mapan777:不知道哪位能告诉我IL-12、IL-12p40、IL-12p35、IL-12p70之间的关系是什么啊?丁香园网友qdwanfeng2007认为:我也正想研究il-12,希望能和你共同探讨一下,P40和P35是P70的两个亚基吧?丁香园网友mapan777认为:是的,它们联合起来起作用,我想做PCR,但是快被这个引物折磨死了,不知楼上是要做什么?丁香园网友qdwanfeng2007认为:我想研究研究白介素12的抗肿瘤作用和机理,没大研究过PCR,可能帮不了你。是不是引物
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