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发货周期:现货
博士德生物
cell culture supernates, serum and plasma(heparin, EDTA, citrate).
96T
货号 | EK0413 |
---|---|
产品名称 | Human IL-8 ELISA Kit |
产品描述 | For quantitative detection of human IL-8 in cell culture supernates, serum and plasma(heparin, EDTA, citrate). |
免疫原 | E.coli, S28-S99 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <1pg/ml |
检测范围 | 15.6pg/ml-1000pg/ml(cellculturesupernates)7.8pg/ml-500pg/ml(serum,plasma) |
基因名称 | CXCL8 |
基因全名 | chemokine (C-X-C motif) ligand 8 |
Uniprot ID | P10145 |
指标别名 | (Ala-IL-8)77|(Ser-IL-8)72|3 10C|3-10C|AMCF I|AMCF-I|AMCFI|b ENAP|B-ENAP|Beta-thromboglobulin-like protein|C X C motif chemokine 8|C-X-C motif chemokine 8|Chemokine (C X C motif) ligand 8|CXCL8 |Emoctakin|GCP 1|GCP-1|GCP/IL-8 protein I|GCP/IL-8 protein II|GCP/IL-8 protein III|GCP/IL-8 protein IV|GCP/IL-8 protein V|GCP/IL-8 protein VI|GCP1 |Granulocyte chemotactic protein 1|IL 8|IL-8|IL-8(1-77)|IL-8(9-77)|IL8|IL8/NAP1 form I|IL8/NAP1 form II|IL8/NAP1 form III|IL8/NAP1 form IV|IL8/NAP1 form V|IL8/NAP1 form VI|IL8_HUMAN|Interleukin 8|K60|LECT|LUCT|Lymphocyte derived neutrophil activating factor|Lymphocyte-derived neutrophil-activating factor|LYNAP|MDNCF|MDNCF-b|MDNCF-c|MONAP|Monocyte derived neutrophil activating peptide|Monocyte derived neutrophil chemotactic factor|Monocyte-derived neutrophil chemotactic factor|Monocyte-derived neutrophil-activating peptide|NAF|NAP 1|NAP-1|NAP1|Neutrophil activating peptide 1|Neutrophil activating protein 1|Neutrophil-activating factor|Neutrophil-activating protein 1|Protein 3 10C|Protein 3-10C|SCYB8|Small inducible cytokine subfamily B member 8|T cell chemotactic factor|T-cell chemotactic factor|TSG 1|TSG-1|TSG1 |
背景资料 | Interleukin-8, also called neutrophil-activating peptide-1 or SCYB8, is a tissue-derived peptide secreted by several types of cells in response to inflammatory stimuli. Monocyte-derived neutrophil chemotactic factor(MDNCF/IL-8, suggested gene symbol IL8) is a cytokine that chemoattracts and activates neutrophils. IL-8 is produced and released from human adipose tissue and from isolated adipocytes in vitro, which may indicate that IL-8 from adipose tissue could be involved in some of the obesity-related complications. The MDNCF/IL-8 gene is placed on the human gene map at position 4q12-q21. This is the same location where at least three other members(platelet factor 4, melanoma growth stimulatory activity, and interferon-gamma induced factor) of the platelet factor 4 gene superfamily reside. Human IL-8 consists of 99 amino acids in precursor form and 79 amino acids in mature form. |
显色时间 | 15-20分钟 |
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK0413-CAP | 96-well plate precoated with anti- human IL-8 antibody | 1 |
EK0413-ST | Lyophilized recombinant human IL-8 standard | 10ng/tubex2 |
EK0413-DA | Biotinylated anti- human IL-8 antibody | 100ul(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 100ul(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 16 | 66 | 234 | 16 | 63 | 230 |
Standard deviation | 0.72 | 2.83 | 14.27 | 0.8 | 3.84 | 17.48 |
CV(%) | 4.5% | 4.3% | 6.1% | 5% | 6.1% | 7.6% |
Concentration(pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
O.D. | 0.003 | 0.170 | 0.321 | 0.594 | 1.121 | 1.878 | 2.552 | 2.779 |
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