手机验证
发货周期:现货
博士德生物
cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).
Mouse
96T
货号 | EK0408 |
---|---|
产品名称 | Mouse IL-5 ELISA Kit |
产品描述 | For quantitative detection of mouse IL-5 in cell culture supernates and serum. |
免疫原 | NSO, M21-G133 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <2pg/ml |
检测范围 | 15.6pg/ml-1000pg/ml |
基因名称 | IL5 |
基因全名 | interleukin 5 |
Uniprot ID | P04401 |
指标别名 | B cell differentiation factor I|B-cell differentiation factor I|Colony stimulating factor eosinophil|EDF|Eosinophil differentiation factor|IL 5|IL-5|IL5|IL5_HUMAN|Interleukin 5|Interleukin 5 (colony stimulating factor eosinophil)|Interleukin-5|Interleukin5|T cell replacing factor|T-cell replacing factor|TRF |
背景资料 | Interleukin-5(IL-5) is also known as eosinophil differentiation factor(EDF). IL-5 is a potential candidate gene in the pathogenesis of asthma, as it is the main cytokine controlling eosinophil activity and eosinophils are pivotal in the development of airway inflammation. The predicted amino acid sequence of 134 amino acids is identical with that recently reported for human interleukin-5 but shows no significant homology with other known hemopoietic growth regulators. IL-5 is a lineage-specific hematopoietic growth factor that stimulates the production of eosinophils and eosinophil colonies from normal human bone marrow cells. IL-5 gene to human chromosome 5 at bands q23-31. Human and mouse of IL-5 share 70% amino acid sequence homology. |
显色时间 | 15-20分钟 |
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK0408-CAP | 96-well plate precoated with anti- mouse IL-5 antibody | 1 |
EK0408-ST | Lyophilized recombinant mouse IL-5 standard | 10ng/tubex2 |
EK0408-DA | Biotinylated anti- mouse IL-5 antibody | 100ul(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 100ul(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
1. 96孔读板仪器。
2. 自动洗板机.
3. 移液器和枪头。建议使用多通道移液器/排枪。
4. Eppendorf 管。
5. 额外洗板缓冲液 (neutral PBS 或 TBS。试剂盒内提供足量。如需额外缓冲液可参考如下配方)。
0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
*样品准备的相关试剂也不含在试剂盒中。Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 36 | 220 | 453 | 34 | 214 | 478 |
Standard deviation | 2.52 | 9.24 | 21.29 | 2.58 | 10.7 | 30.59 |
CV(%) | 7% | 4.2% | 4.7% | 7.6% | 5% | 6.4% |
Concentration(pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
O.D. | 0.025 | 0.083 | 0.144 | 0.258 | 0.467 | 0.882 | 1.754 | 2.352 |
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