- 询价
- Biotium
- 美国
- 31038
- 2026年01月16日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
4°C
- 保质期：
6个月
- 英文名：
Enhancer for Gram Negative Bacteria, 5X Solution 31038
- 库存：
99
- 供应商：
Biotium
- CAS号：
1
Enhancer for Gram Negative Bacteria improves - and xx™-mediated discrimination between live and dead gram-negative bacteria.
- Used in viability PCR with or xx™
- Decreases dead cell signal in gram-negative bacteria
- Should not be used with gram-positive bacteria
- Provided as a 5X solution
The mechanism by which Enhancer improves live/dead discrimination has not been determined. It may improve passive permeability of dead cell walls or membranes to the dye, and/or improve access of the dye to the dead cell DNA.
Viability PCR
Viability PCR is a powerful technology for the sensitive and rapid detection of viable microorganisms. Unlike time-consuming culturing methods, qPCR is a fast and sensitive method of detection. However, normal qPCR does not distinguish between live and dead cells. With v-PCR using xx™ or, you get the speed, sensitivity and specificity of PCR, plus quantifiable viability. And because no culturing is required, you can even detect viable but not culturable (VBNC) bacteria. The v-PCR technology can be applied not only to bacteria but to other organisms like yeast, viruses, eukaryotes, and archaea.
To learn more about the advantages of determining microbial or cell viability using viability PCR, visit the Viability PCR Technology Page.
xx technology is covered by granted and/or pending US and international patents.
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文献和实验and will remain blue-violet in appearance. Gram negative bacteria, however, take on a pink color and are easily distinguishable from the Gram positives. Again, rinse with water for 5 seconds to remove any excess of dye. After you have completed steps
Permeabilization of gram-negative bacteria with KPi/hexane for the release of L-asparaginase
Permeabilization of gram-negative bacteria with KPi/hexane for the release of L-asparaginase Source: Hikmet Geckil's Lab Abstract: This is a fast, efficient
positive cells will incorporate little or no counterstain and will remain blue-violet in appearance. Gram negative bacteria, however, take on a pink color and are easily distinguishable from the Gram positives. Again, rinse with water for 5 seconds
