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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名:
CD28 Monoclonal Antibody (CD28.2), Functional Grade, eBioscience™
- 浓度:
1 mg/mL
- 宿主:
Mouse
- 供应商:
嵘崴达
- 保质期:
见产品说明书
- 库存:
需确认
- 级别:
见产品说明书
- 适应物种:
见产品说明书
- 标记物:
见产品说明书
- 是否单克隆:
单克隆
- 保存条件:
4° C
- 亚型:
IgG1
- 规格:
500 ug
货号 16-0289-85
产品描述
Description: The CD28.2 monoclonal antibody reacts with the human CD28 molecule, a 44 kDa homodimer expressed by thymocytes, mature T cells and plasma cells. CD28 is a ligand for CD80 (B7-1) and CD86 (B7-2) and is a potent co-stimulator of T cells. Signaling through CD28 augments IL-2 and IL-2 receptor expression as well as cytotoxicity of CD3-activated T cells.
Applications Reported: The CD28.2 antibody has been reported for use in flow cytometric analysis. CD28.2 has also been reported in costimulation of T cells in in vitro functional assays.
Applications Tested: TheCD28.2 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Storage and handling: Use in a sterile environment.
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文献和实验7211011 小鼠淋巴细胞分离液 达优 100339 Ultra-LEAF™ Purified anti-mouse CD3ε Antibody BioLegend 102115 Ultra-LEAF™ Purified anti-mouse CD28 Antibody BioLegend CD3/CD28 Streptamer® 多聚体法: 货号 名称
transfer of residual antibody. Vials of Dynabeads® CD3⁄CD28 CTS™ are at negative pressure – liquid must be displaced with air and vice versa. 2) Place one 10 ml vial of Dynabeads® CD3⁄CD28 CTS™ on the MPC-1 for 1 min. 3)
Dual- and Triple-Color Fluorospot
functional antibody pairs are derived from a few species. Another strategy being employed for triple staining is the use of additional tag/anti-tag systems other than FITC/anti-FITC (Fig. 1). 实验试剂 1. Blocking reagent after coating: 10
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