IFN gamma Monoclonal Antibody

IFN gamma Monoclonal Antibody (NIB42), eBioscience™

货号 14-7318-85

产品描述
Description: The NIB42 antibody reacts with human interferon-gamma (IFN-gamma). The NIB42 antibody is a neutralizing antibody. IFN-gamma is an ~20 kDa factor produced by activated T, B and NK cells and is an anti-viral and anti-parasitic cytokine. IFN-gamma in synergy with other cytokines, such as TNF-alpha, inhibits proliferation of normal and transformed cells. Immunomodulatory effects of IFN-gamma are exerted on a wide range of cell types expressing the high affinity receptors for IFN-gamma. Glycosylation of IFN-gamma does not affect its biological activity.

Applications Reported: The NIB42 antibody has been reported for use in capture of human IFN gamma by ELISA, and for neutralizing IFN gamma bioactivity. Use Functional Grade purified for functional assays cat. 16-7318).

Applications Tested: The NIB42 antibody has been tested as the capture antibody in a sandwich ELISA for analysis of human Interferon-gamma (IFN-g) in combination with the biotin 4S.B3 (13-7319) antibody for detection and recombinant human IFNg (29-8319) as the standard. A suitable range of concentrations of this antibody for ELISA capture is 2-8 µg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 1000 pg/mL - 8 pg/mL should be included in each ELISA plate.For ELISPOT Capture, the alternative clone MD-1 is recommended.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

上海嵘崴达实业有限公司，专业经营生命科学领域的科研试剂、仪器和实验室消耗品，
致力于向国内生命科学研究人员提供合格产品和完善服务。
主要经营的试剂品牌：
Roche试剂，部分常用现货，周三定货，2-3周到货。
GE试剂，耗材，部分常用现货，周二，周五定货，3周左右。
SIGMA试剂，周一，五定货，3周左右到货。
R&D试剂，周一定货，3-4周到货。
THERMO试剂，部分现货，周三定货，2-3周到货。
ABCAM试剂，国内现货三天左右，期货四周左右。
Peprotech试剂，部分常用现货，周三定货，2-3周到货。
罗氏原料，请来电来邮咨询。
OXOID，BD培养基础货期八周左右，部分现货。
MCE激动剂，ACROS、ALFA、阿拉丁等生化试剂现货定购。