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- eBioscience
- 12-0049-42
- 原装进口
- 2025年09月09日
- Mouse
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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名：
CD4 Monoclonal Antibody (RPA-T4), PE, eBioscience™
- 浓度：
5 µL/Test
- 宿主：
Mouse
- 供应商：
嵘崴达
- 保质期：
见产品说明书
- 抗原来源：
见产品说明书
- 适应物种：
见产品说明书
- 目录编号：
12-0049-42
- 库存：
需确认
- 标记物：
见产品说明书
- 是否单克隆：
单克隆
- 保存条件：
见产品说明书
- 亚型：
IgG1
- 规格：
100 tests
货号 12-0049-42
产品描述
Description: The RPA-T4 monoclonal antibody reacts with human CD4, a 59 kDa cell surface receptor expressed by a majority of thymocytes, subpopulation of mature T cells (T-helper cells) and in low levels on monocytes. CD4 is a receptor for the human immunodeficiency virus (HIV). RPA-T4 blocks HIV binding and mixed lymphocyte reaction. The RPA-T4 antibody recognizes a different epitope than the OKT4 monoclonal antibody, and these antibodies do not cross-block binding to each other's respective epitopes.
Applications Reported: The RPA-T4 antibody has been reported for use in flow cytometric analysis.
Applications Tested: The FITC conjugated RPA-T4 is offered in 2 formats:
- µg size: has been tested by flow cytometric analysis and can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
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文献和实验Native Chromatin Preparation and Illumina/Solexa Library Construction
Antibody and preimmune serum for ChIP CD4+ T cell Isolation Kit II (human) (Miltenyi Biotec) Alternatively, Dynabeads Untouched Human CD4 T Cells Kit (Invitrogen) can be used (see Steps 5-21). DNA End-Repair Kit (Epicentre Biotechnologies
Intracellular Cytokine Staining Protocol
mature and developing T cells T helper, CD4+ CD4 RPA-T4 14-0049 mature and developing T cells T regulatory, CD4+CD
secondary antibody review -- data from 99 publications
cytometry used as a control to detect cell responses targeted antigen 7 Alexa Fluor 488 7 Cy3 8 goat IgG Alexa Fluor 488 1:2000 detect antibody binding in human embryonic kidney 293T cells Invitrogen 9 donkey
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